CRMP1 Polyclonal antibody

CRMP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10317-1-AP

Synonyms

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3



Tested Applications

Positive WB detected inmouse lung tissue, mouse brain tissue, rat lung tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10317-1-AP targets CRMP1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRMP1 fusion protein Ag0369
Full Name collapsin response mediator protein 1
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC000252
Gene Symbol CRMP1
Gene ID (NCBI) 1400
RRIDAB_2085347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62-kDa protein and its 75-kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

Protocols

Product Specific Protocols
WB protocol for CRMP1 antibody 10317-1-APDownload protocol
IHC protocol for CRMP1 antibody 10317-1-APDownload protocol
IF protocol for CRMP1 antibody 10317-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Chem Biol Interact

Exposure to Benzo(a)pyrene damages mitochondrial function via suppressing mitochondrial melatonin receptors in ovarian corpus luteum during early pregnancy

Authors - Jie Yang
ratWB

Molecules

Senescent Phenotype of Astrocytes Leads to Activation of BV2 Microglia and N2a Neuronal Cells Death

Authors - Wenyou Zhang
mouse,humanWB,CoIP

Neuron

Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development

Authors - Minjie Shen