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CSK Monoclonal antibody

CSK Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B12C5

Cat no : 66115-1-Ig

Synonyms

C Src kinase, c src tyrosine kinase, CSK, Protein tyrosine kinase CYL, Tyrosine protein kinase CSK

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of Jurkat using 66115-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of RAW 264.7 using 66115-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of NIH/3T3 using 66115-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CSK Monoclonal antibody (66115-1-Ig)

    WB analysis of HeLa using 66115-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66115-1-Ig (CSK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CSK Monoclonal antibody (66115-1-Ig)

    IF Staining of A431 using 66115-1-Ig

    Immunofluorescent analysis of A431 cells using 66115-1-Ig (CSK antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, NIH/3T3 cells, RAW 264.7 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 66115-1-Ig is CSK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CSK fusion protein Ag12103
Full Name c-src tyrosine kinase
Calculated Molecular Weight 450 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC106073
Gene Symbol CSK
Gene ID (NCBI) 1445
RRIDAB_2881514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701).

Protocols

Product Specific Protocols
WB protocol for CSK antibody 66115-1-IgDownload protocol
IF protocol for CSK antibody 66115-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Andrology

Trps1 targets Ccnd1 to regulate mouse Leydig cell proliferation.

Authors - Jiandong Sun
View Article