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CSNK1G1 Polyclonal antibody

CSNK1G1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16384-1-AP

Synonyms

casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CSNK1G1 Polyclonal antibody (16384-1-AP)

    WB analysis using 16384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CSNK1G1 Polyclonal antibody (16384-1-AP)

    WB analysis of Jurkat using 16384-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using CSNK1G1 Polyclonal antibody (16384-1-AP)

    WB analysis of COS-7 using 16384-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CSNK1G1 Polyclonal antibody (16384-1-AP)

    IP experiment of Jurkat using 16384-1-AP

    IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using CSNK1G1 Polyclonal antibody (16384-1-AP)

    IHC staining of human liver using 16384-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CSNK1G1 Polyclonal antibody (16384-1-AP)

    IHC staining of human liver using 16384-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, COS-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16384-1-AP targets CSNK1G1 in WB, IP, IHC, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSNK1G1 fusion protein Ag9395
Full Name casein kinase 1, gamma 1
Calculated Molecular Weight 422 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC017236
Gene Symbol CSNK1G1
Gene ID (NCBI) 53944
RRIDAB_2087300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.

Protocols

Product Specific Protocols
WB protocol for CSNK1G1 antibody 16384-1-APDownload protocol
IHC protocol for CSNK1G1 antibody 16384-1-APDownload protocol
IP protocol for CSNK1G1 antibody 16384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Immunol

Casein Kinase 1γ1 Inhibits the RIG-I/TLR Signaling Pathway through Phosphorylating p65 and Promoting Its Degradation.

Authors - Yetao Wang
View Article