• Featured Product
  • KD/KO Validated

CUL4B Antibody

CUL4B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12916-1-AP

Synonyms

CUL 4B, CUL4B, cullin 4B, KIAA0695, MRXHF2, MRXSC, SFM2



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells , mouse testis tissue, human testis tissue, SH-SY5Y cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12916-1-AP targets CUL4B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity dog, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL4B fusion protein Ag3563
Full Name cullin 4B
Calculated molecular weight 913aa,104 kDa
Observed molecular weight 102 kDa
GenBank accession number BC036216
Gene symbol CUL4B
Gene ID (NCBI) 8450
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

Protocols

Product Specific Protocols
WB protocol for CUL4B antibody 12916-1-APDownload protocol
IHC protocol for CUL4B antibody 12916-1-APDownload protocol
IF protocol for CUL4B antibody 12916-1-APDownload protocol
IP protocol for CUL4B antibody 12916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.

Authors - Haitao Sun
humanIF

Oncogenesis

CUL4B promotes prostate cancer progression by forming positive feedback loop with SOX4.

Authors - Mei Qi
humanWB

Sci Rep

Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.

Authors - Tsai-Chun Lai
  • KD Validated
humanWB

Cells

CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy.

Authors - Ssu-Ju Fu
humanWB

Signal Transduct Target Ther

The CRL3BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration.

Authors - Lihui Li
humanWB

J Biol Chem

Dysregulation of CUL4A and CUL4B Ubiquitin Ligases in Lung Cancer.

Authors - Lei Jia