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CUL4B Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 12916-1-AP

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Con: 46 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

CUL4B Antibody IHC human gliomas tissue 12916-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12916-1-AP( CUL4B antibody) at dilution of 1:100 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CUL4B Antibody IHC human gliomas tissue 12916-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12916-1-AP( CUL4B antibody) at dilution of 1:100 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CUL4B Antibody WB HeLa cells 12916-1-AP

WB result of CUL4B antibody (12916-1-AP, 1:2,000) with si-Control and si-CUL4B transfected HeLa cells.

CUL4B Antibody IP HeLa cells 12916-1-AP

IP Result of anti-CUL4B (IP:12916-1-AP, 3ug; Detection:12916-1-AP 1:1000) with HeLa cells lysate 4650ug.

CUL4B Antibody IF HepG2 cells 12916-1-AP

Immunofluorescent analysis of HepG2 cells, using CUL4B antibody 12916-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

CUL4B Antibody WB HEK-293 cells 12916-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12916-1-AP( CUL4B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, dog, rat
Positive WB detected in:
HEK-293 cells, HeLa cells , mouse testis tissue, human testis tissue, SH-SY5Y cells, HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human gliomas tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CUL4B fusion protein Ag3563
Full name:
cullin 4B
Calculated molecular weight:
913aa,104 kDa
Observed molecular weight:
102 kDa
GenBank accession number:
BC036216
Gene ID (NCBI):
8450
Gene symbol
CUL4B
Synonyms
CUL 4B, CUL4B, cullin 4B, KIAA0695, MRXHF2, MRXSC, SFM2

Background

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).


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