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- KD/KO Validated
CUL4B Polyclonal antibody
CUL4B Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 12916-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells , mouse testis tissue, human testis tissue, SH-SY5Y cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 28 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
12916-1-AP targets CUL4B in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | canine, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CUL4B fusion protein Ag3563 |
| Full Name | cullin 4B |
| Calculated molecular weight | 913 aa, 104 kDa |
| Observed molecular weight | 102 kDa |
| GenBank accession number | BC036216 |
| Gene symbol | CUL4B |
| Gene ID (NCBI) | 8450 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CUL4B antibody 12916-1-AP | Download protocol |
| IHC protocol for CUL4B antibody 12916-1-AP | Download protocol |
| IF protocol for CUL4B antibody 12916-1-AP | Download protocol |
| IP protocol for CUL4B antibody 12916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Genet Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2. | ||
Oncogenesis CUL4B promotes prostate cancer progression by forming positive feedback loop with SOX4. | ||
Sci Rep Regulation of liver receptor homologue-1 by DDB2 E3 ligase activity is critical for hepatic glucose metabolism.
| ||
Cells CUL4-DDB1-CRBN E3 Ubiquitin Ligase Regulates Proteostasis of ClC-2 Chloride Channels: Implication for Aldosteronism and Leukodystrophy. | ||
Signal Transduct Target Ther The CRL3BTBD9 E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration. | ||
