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CXCR4 Antibody

CXCR4 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

4B5E4

Cat no : 60042-1-Ig

Synonyms

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM



Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, U-937 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

60042-1-Ig targets CXCR4 in WB, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen CXCR4 fusion protein Ag1528
Full Name chemokine (C-X-C motif) receptor 4
Calculated molecular weight 352aa,40 kDa
Observed molecular weight 60~70 kDa
GenBank accession number BC020968
Gene symbol CXCR4
Gene ID (NCBI) 7852
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Protocols

Product Specific Protocols
WB protocol for CXCR4 antibody 60042-1-IgDownload protocol
FC protocol for CXCR4 antibody 60042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

The overexpression of GRASP might inhibit cell proliferation and invasion in hepatocellular carcinoma.

Authors - Yang Cheng
mouseWB

Evid Based Complement Alternat Med

Combination of Ligusticum Chuanxiong and Radix Paeonia Promotes Angiogenesis in Ischemic Myocardium through Notch Signalling and Mobilization of Stem Cells.

Authors - Wei-Li Shi
WB

Mol Med Rep

Taspine derivative TAS9 regulates cell growth and metastasis of human hepatocellular carcinoma.

Authors - Rui Liu
mouseWB

Cytotechnology

Co-culture with TM4 cells enhances the proliferation and migration of rat adipose-derived mesenchymal stem cells with high stemness.

Authors - Yanxia Luo
humanWB

Scand J Gastroenterol

CXCR7 as a chemokine receptor for SDF-1 promotes gastric cancer progression via MAPK pathways.

Authors - Ameng Shi
humanWB

Inflammation

Porphyromonas gingivalis-Induced MIF Regulates Intercellular Adhesion Molecule-1 Expression in EA.hy926 Cells and Monocyte-Endothelial Cell Adhesion Through the Receptors CD74 and CXCR4.

Authors - Yun Wu