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  • KD/KO Validated

CYLD Polyclonal antibody

CYLD Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11110-1-AP

Synonyms

CDMT, CYLD, CYLD1, CYLDI, Deubiquitinating enzyme CYLD, EAC, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, Ubiquitin thiolesterase CYLD, USPL2



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, A431 cells, Jurkat cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue, human brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11110-1-AP targets CYLD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYLD fusion protein Ag1598
Full Name cylindromatosis (turban tumor syndrome)
Calculated molecular weight 107 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC012342
Gene symbol CYLD
Gene ID (NCBI) 1540
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CYLD, also named as CYLD1, belongs to the peptidase C67 family. It is the protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. CYLD has endodeubiquitinase activity and plays an important role in the regulation of pathways leading to NF-kappa-B activation. CYLD contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. It is a negative regulator of Wnt signaling. CYLD inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. CYLD plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. It is required for normal cell cycle progress and normal cytokinesis. CYLD inhibits nuclear translocation of NF-kappa-B and plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. It is dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. CYLD negatively regulates TNFRSF11A signaling and osteoclastogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human CYLD.

Protocols

Product Specific Protocols
WB protocol for CYLD antibody 11110-1-APDownload protocol
IHC protocol for CYLD antibody 11110-1-APDownload protocol
IP protocol for CYLD antibody 11110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Chem Biol Interact

miR-501 is upregulated in cervical cancer and promotes cell proliferation, migration and invasion by targeting CYLD.

Authors - Jaceline Sanches
  • KD Validated
humanWB

Oncol Lett

Downregulation of miR-362-5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells.

Authors - Fang Ni
humanWB

Int J Clin Exp Pathol

Down-regulation of miR-181b promotes apoptosis by targeting CYLD in thyroid papillary cancer.

Authors - Dengfeng Li
mouseWB,IHC

J Dev Orig Health Dis

Maternal folic acid depletion during early pregnancy increases sensitivity to squamous tumor formation in the offspring in mice.

Authors - Tomoyo Kawakubo-Yasukochi
humanWB

BMB Rep

MicroRNA-301b promotes cell proliferation and apoptosis resistance in triple-negative breast cancer by targeting CYLD.

Authors - Hongming Song
  • KD Validated
humanWB

Mediators Inflamm

CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells.

Authors - Xing Lin
  • KD Validated