HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP( Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (cleaved-caspase 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 25546-1-AP( Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP( Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP( Caspase 3 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP( Caspase 3 Antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-Caspase 3 (IP:25546-1-AP, 4ug; Detection:25546-1-AP 1:300) with Jurkat cells lysate 3200ug.

Immunofluorescent analysis of HepG2 cells using 25546-1-AP( Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 HeLa cells were stained with 0.2ug Caspase 3 antibody (25546-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.