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cleaved-caspase 3 Antibody 97 Publications

Rabbit Polyclonal| Catalog number: 25546-1-AP

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Con: 48 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

cleaved-caspase 3 Antibody WB Tunicamycin treated HepG2 cells 25546-1-AP

Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP( Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

cleaved-caspase 3 Antibody IP Jurkat cells 25546-1-AP

IP Result of anti-Caspase 3 (IP:25546-1-AP, 4ug; Detection:25546-1-AP 1:300) with Jurkat cells lysate 3200ug.

cleaved-caspase 3 Antibody IHC human skeletal muscle tissue 25546-1-AP

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP( Caspase 3 Antibody) at dilution of 1:50 (under 10x lens)

cleaved-caspase 3 Antibody IHC human skeletal muscle tissue 25546-1-AP

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP( Caspase 3 Antibody) at dilution of 1:50 (under 40x lens)

cleaved-caspase 3 Antibody IF HepG2 cells 25546-1-AP

Immunofluorescent analysis of HepG2 cells using 25546-1-AP( Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

cleaved-caspase 3 Antibody FC HeLa cells 25546-1-AP

1X10^6 HeLa cells were stained with 0.2ug Caspase 3 antibody (25546-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, hamster, sheep
Positive WB detected in:
Tunicamycin treated HepG2 cells, Tunicamycin treated HeLa cells, Staurosporine treated Jurkat cells
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight:
32 kDa
Observed molecular weight:
17 kDa
GenBank accession number:
NM_032991
Gene ID (NCBI):
836
Gene symbol
CASP3
Synonyms

Background

Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.


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