cleaved-caspase 3 Antibody 72 Publications

Rabbit Polyclonal| Catalog number: 25546-1-AP

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Freight/Packing

Con: 48 μg/150 μl

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Species specificity:
human, mouse, rat, hamsters, sheep

Positive WB detected in:
Tunicamycin treated HepG2 cells,Tunicamycin treated HeLa cells, Staurosporine treated Jurkat cells

Positive IP detected in:
Jurkat cells

Positive IHC detected in:
human skeletal muscle tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
caspase 3, apoptosis-related cysteine peptidase

Calculated molecular weight:
32 kDa

Observed molecular weight:
17 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CASP3

Synonyms
Background

Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.


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