DDX4,VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17545-1-AP

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17545-1-AP is DDX4,VASA Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX4,VASA fusion protein Ag11647
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Calculated Molecular Weight690aa,76 kDa; 724aa,79 kDa
Observed Molecular Weight79 kDa
GenBank Accession NumberBC047455
Gene Symbol DDX4
Gene ID (NCBI) 54514
RRIDAB_2918053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Protocols

Product Specific Protocols
WB protocol for DDX4,VASA antibody 17545-1-APDownload protocol
IHC protocol for DDX4,VASA antibody 17545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Sci Rep

FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells.

Authors - Larissa Zarate-Garcia