DFF45/DFFA Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 10191-2-AP

Featured Product KD/KO validated

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Con: 60 μg/150 μl

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Species specificity:

Positive WB detected in:
HEK-293 cells, A549 cells, HeLa cells

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human prostate cancer tissue

Positive IF detected in:
HEK-293 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Purification method:
Antigen affinity purification


PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Full name:
DNA fragmentation factor, 45kDa, alpha polypeptide

Calculated molecular weight:
37 kDa

Observed molecular weight:
45 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol

DFF 45, DFF1, DFF45, DFF45/DFFA, DFFA, ICAD, Inhibitor of CAD

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.

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