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  • Featured Product
  • KD/KO Validated

DFNA5/GSDME Polyclonal antibody

DFNA5/GSDME Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13075-1-AP

Synonyms

Cleaved Gasdermin E, deafness, autosomal dominant 5, DFNA5, DFNA5/GSDME, Gasdermin E, Gasdermin-E, GSDME, ICERE 1, ICERE1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    WB analysis using 13075-1-AP

    Untreated, and etoposide treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    WB analysis using 13075-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    WB analysis of A549 using 13075-1-AP

    WB result of DFNA5/ GSDME antibody (13075-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DFNA5/ GSDME transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    WB analysis using 13075-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    WB analysis of Y79 using 13075-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    IP experiment of SH-SY5Y using 13075-1-AP

    IP result of anti-DFNA5/GSDME (IP:13075-1-AP, 4ug; Detection:13075-1-AP 1:15000) with SH-SY5Y cells lysate 1240 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    IHC staining of mouse brain using 13075-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    IHC staining of mouse brain using 13075-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    IHC staining of mouse small intestine using 13075-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    IHC staining of mouse small intestine using 13075-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using DFNA5/GSDME Polyclonal antibody (13075-1-AP)

    FC experiment of SH-SY5Y using 13075-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DFNA5/GSDME (13075-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, Y79 cells, SH-SY5Y cells, HeLa cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13075-1-AP targets DFNA5/GSDME in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DFNA5/GSDME fusion protein Ag3746
Full Name deafness, autosomal dominant 5
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC019689
Gene Symbol DFNA5
Gene ID (NCBI) 1687
RRIDAB_2093053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME, is a protein made of a 31 kDa N-terminus (GSDMD-N) and a 22 kDa C-terminus (GSDMD-C) connected by a peptide linker. Upon activation, the linker is cleaved to separate GsdmD-N from its autoinhibitory domain, GSDMD-C. GSDMD-N forms a transmembrane pore that releases cytokines such as interleukin IL-1β and IL-18 and also disturbs the regulation of ions and water, eventually resulting in pyroptosis.

Protocols

Product Specific Protocols
WB protocol for DFNA5/GSDME antibody 13075-1-APDownload protocol
IHC protocol for DFNA5/GSDME antibody 13075-1-APDownload protocol
IP protocol for DFNA5/GSDME antibody 13075-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,CoIP

Cell Res

Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P

Authors - Yuan-Li Ai
View Article
humanIF

Acta Pharm Sin B

A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer

Authors - Yang Liu
View Article
mouseIF

Cell Death Differ

GSDME-mediated pyroptosis promotes inflammation and fibrosis in obstructive nephropathy.

Authors - Yinshuang Li
View Article

Cell Death Dis

TNFSF15 inhibits progression of diabetic retinopathy by blocking pyroptosis via interacting with GSDME

Authors - Hongkun Zhao
View Article
humanWB

J Mater Chem B

A hybrid membrane coating nanodrug system against gastric cancer via the VEGFR2/STAT3 signaling pathway

Authors - Ying Long
View Article
humanWB

Free Radic Biol Med

Ferroptosis plays a crucial role in lung cell damage caused by ventilation stretch

Authors - Wei Jiang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

James (Verified Customer) (11-18-2022)

Used for GSDME detection in splenocyte extracts

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Spleen
DFNA5/GSDME Antibody Western Blot validation (1/1000 dilution) in Spleen (Cat no:13075-1-AP)
FH

Evelina (Verified Customer) (03-14-2022)

Good antibody to detect GSDME in cell lysates. Although does detect one of the proteins within the ladder (approx.15kDa) when using stronger ECL.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
DFNA5/GSDME Antibody Western Blot validation (1:1000 dilution) in (Cat no:13075-1-AP)