|Positive WB detected in||HepG2 cells, mouse lung tissue, Jurkat cells, MCF-7 cells|
|Positive IHC detected in||human liver tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10471-1-AP targets Seladin 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Seladin 1 fusion protein Ag0756|
|Full Name||24-dehydrocholesterol reductase|
|Calculated molecular weight||60 kDa, 516 aa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC004375|
|Gene ID (NCBI)||1718|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The DHCR24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).
Animal Model Exp Med
Dhcr24 activates the PI3K/Akt/HKII pathway and protects against dilated cardiomyopathy in mice.
Reprod Biol Endocrinol
Diets high in selenium and isoflavones decrease androgen-regulated gene expression in healthy rat dorsolateral prostate.
J Bone Miner Metab
3β-Hydroxysterol-Delta24 reductase plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species.
Seladin-1 is a novel lipopolysaccharide (LPS)-responsive gene and inhibits the tumour necrosis factor-alpha production and osteoclast formation in response to LPS.
Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.