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Seladin 1 Polyclonal antibody

Seladin 1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10471-1-AP

Synonyms

DHCR24, Diminuto/dwarf1 homolog, KIAA0018, Nbla03646, Seladin 1, SELADIN1



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HepG2 cells, MCF-7 cells, mouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10471-1-AP targets Seladin 1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Seladin 1 fusion protein Ag0756
Full Name 24-dehydrocholesterol reductase
Calculated molecular weight 60 kDa, 516 aa
Observed molecular weight 60 kDa
GenBank accession numberBC004375
Gene symbol DHCR24
Gene ID (NCBI) 1718
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The DHCR24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

Protocols

Product Specific Protocols
WB protocol for Seladin 1 antibody 10471-1-APDownload protocol
IHC protocol for Seladin 1 antibody 10471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Animal Model Exp Med

Dhcr24 activates the PI3K/Akt/HKII pathway and protects against dilated cardiomyopathy in mice.

Authors - Wei Dong
ratWB

Reprod Biol Endocrinol

Diets high in selenium and isoflavones decrease androgen-regulated gene expression in healthy rat dorsolateral prostate.

Authors - Legg Russell L RL
mouseIHC

J Bone Miner Metab

3β-Hydroxysterol-Delta24 reductase plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species.

Authors - Mirza Rusella R
humanWB

Immunology

Seladin-1 is a novel lipopolysaccharide (LPS)-responsive gene and inhibits the tumour necrosis factor-alpha production and osteoclast formation in response to LPS.

Authors - Khuda Imtiaz I-E II
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