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Seladin 1 Polyclonal antibody

Seladin 1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10471-1-AP

Synonyms

DHCR24, Diminuto/dwarf1 homolog, KIAA0018, Nbla03646, Seladin 1, SELADIN1



Tested Applications

Positive WB detected inHepG2 cells, mouse lung tissue, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10471-1-AP targets Seladin 1 in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Seladin 1 fusion protein Ag0756
Full Name 24-dehydrocholesterol reductase
Calculated molecular weight 60 kDa, 516 aa
Observed molecular weight 50-60 kDa
GenBank accession numberBC004375
Gene symbol DHCR24
Gene ID (NCBI) 1718
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The DHCR24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

Protocols

Product Specific Protocols
WB protocol for Seladin 1 antibody 10471-1-APDownload protocol
IHC protocol for Seladin 1 antibody 10471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Animal Model Exp Med

Dhcr24 activates the PI3K/Akt/HKII pathway and protects against dilated cardiomyopathy in mice.

Authors - Wei Dong
ratWB

Reprod Biol Endocrinol

Diets high in selenium and isoflavones decrease androgen-regulated gene expression in healthy rat dorsolateral prostate.

Authors - Legg Russell L RL
mouseIHC

J Bone Miner Metab

3β-Hydroxysterol-Delta24 reductase plays an important role in long bone growth by protecting chondrocytes from reactive oxygen species.

Authors - Mirza Rusella R
humanWB

Immunology

Seladin-1 is a novel lipopolysaccharide (LPS)-responsive gene and inhibits the tumour necrosis factor-alpha production and osteoclast formation in response to LPS.

Authors - Khuda Imtiaz I-E II
  • KD Validated
human,mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu