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DHX32 Polyclonal antibody

DHX32 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19808-1-AP

Synonyms

DDX32, DEAD/H box 32, DEAD/H helicase like protein 1, DEAH box protein 32, DHLP1, DHX32, HuDDX32



Tested Applications

Positive WB detected inmouse kidney tissue, mouse colon tissue, A431 cells, COLO 320 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19808-1-AP targets DHX32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHX32 fusion protein Ag13834
Full Name DEAH (Asp-Glu-Ala-His) box polypeptide 32
Calculated Molecular Weight 743 aa, 84 kDa
Observed Molecular Weight 84 kDa, 75 kDa
GenBank Accession NumberBC002473
Gene Symbol DHX32
Gene ID (NCBI) 55760
RRIDAB_10641031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHX32, a new member of the DEAH subfamily of DEAD box proteins, is a novel putative RNA helicase with an activation-dependent paten of expression and characterized by a conserved helicase domain consisting of 7 to 8 conserved motifs. RNA helicases of the DEAD box family are highly conserved enzymes that are required for RNA metabolism from transcription to degradation and are therefore important players in gene expression. DHX32 may play an important role in the development of colorectal cancer. This is a rabbit polyclonal anti-DHX32 antibody raised against part of the C-terminal chain of human DHX32.

Protocols

Product Specific Protocols
WB protocol for DHX32 antibody 19808-1-APDownload protocol
IHC protocol for DHX32 antibody 19808-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncol Lett

DHX32 expression is an indicator of poor breast cancer prognosis.

Authors - Meng Wang
humanWB,IHC

Transl Cancer Res

Silencing of DHX32 increases the proliferation of liver cancer cells.

Authors - Min-Jing Cai
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