Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10098-2-AP targets DHX38 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0144 Product name: Recombinant human DHX38 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-280 aa of BC004235 Sequence: DCQVGGLICKSKSAASEQHVFKAPAPRPSLLGLDLLASLKRREREEKDDGEDKKKSKVSSYKDWEESKDDQKDAEEEGGDQAGQNIRKDRHYRSARVETPSHPGGVSEEFWERSRQRERERREHGVYASSKEEKDWKKEKSRDRDYDRKRDRDERDRSRHSSRSERDGGSERSSRRNEPESPRHRPKDAATPSRSTWEEEDSGYGSSRRSQWESPSPTPSYRDSERSHRLSTRDRDRSVRGKYSDDTPLPTPSYKYNEWADD Predict reactive species |
| Full Name | DEAH (Asp-Glu-Ala-His) box polypeptide 38 |
| Calculated Molecular Weight | 141 kDa |
| Observed Molecular Weight | 141 kDa |
| GenBank Accession Number | BC004235 |
| Gene Symbol | DHX38 |
| Gene ID (NCBI) | 9785 |
| RRID | AB_2092294 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92620 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DHX38 antibody 10098-2-AP | Download protocol |
| IP protocol for DHX38 antibody 10098-2-AP | Download protocol |
| WB protocol for DHX38 antibody 10098-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DHX38 Polyclonal antibody (Cat# 10098-2-AP) has 9 citations across journals including Gastroenterology, Nature Cell Biology, Stem Cells, Cell Signaling, iScience, Frontiers in Molecular Biosciences, PLoS ONE, FEBS Open Bio, and Hematology. Research fields span pre-mRNA splicing, epigenetics in germ cells, cancer biology (NSCLC, ovarian clear cell carcinoma, leukemia), retinal development, ribosomal biogenesis, stem cell reprogramming, and bile acid transport in cholestasis.
| Species | Application | Title |
|---|---|---|
Gastroenterology Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis. | ||
Nat Cell Biol Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. | ||
Stem Cells Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming. | ||
Cell Signal DHX38 enhances proliferation, metastasis, and EMT progression in NSCLC through the G3BP1-mediated MAPK pathway
| ||
iScience The splicing factor DHX38 enables retinal development through safeguarding genome integrity
| ||
Front Mol Biosci Split-APEX implicates splicing factor SRSF1 and splicing helicases in ribosomal biogenesis. |
