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- KD/KO Validated
DICER1 Antibody
DICER1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20567-1-AP
Synonyms
DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, HepG2 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human testis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
Product Information
20567-1-AP targets DICER1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | dicer 1, ribonuclease type III |
| Calculated molecular weight | 219 kDa |
| Observed molecular weight | 220-250 kDa, 90 kDa |
| GenBank accession number | NM_030621 |
| Gene symbol | DICER1 |
| Gene ID (NCBI) | 23405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DICER1 antibody 20567-1-AP | Download protocol |
| IHC protocol for DICER1 antibody 20567-1-AP | Download protocol |
| IF protocol for DICER1 antibody 20567-1-AP | Download protocol |
| IP protocol for DICER1 antibody 20567-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Pseudogene RACGAP1P activates RACGAP1/Rho/ERK signalling axis as a competing endogenous RNA to promote hepatocellular carcinoma early recurrence. | ||
Oncotarget Dicer suppresses MMP-2-mediated invasion and VEGFA-induced angiogenesis and serves as a promising prognostic biomarker in human clear cell renal cell carcinoma. | ||
FEBS Open Bio LncRNA UCA1 is necessary for TGF-β-induced epithelial-mesenchymal transition and stemness via acting as a ceRNA for Slug in glioma cells. | ||
Am J Cancer Res Imbalance of a KLF4-miR-7 auto-regulatory feedback loop promotes prostate cancer cell growth by impairing microRNA processing. | ||
Cell Physiol Biochem Iridoids with Genipin Stem Nucleus Inhibit Lipopolysaccharide-Induced Inflammation and Oxidative Stress by Blocking the NF-κB Pathway in Polycystic Ovary Syndrome. | ||
J Virol The Capsid Protein of Semliki Forest Virus Antagonizes RNA Interference in Mammalian Cells. |
