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DICER1 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 20567-1-AP

Featured Product KD/KO validated

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Con: 85 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

DICER1 Antibody WB HepG2 cells 20567-1-AP

WB result of DICER1 antibody (20567-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-DICER1 transfected HepG2 cells

DICER1 Antibody WB HeLa cells 20567-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 20567-1-AP (DICER1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

DICER1 Antibody IP Jurkat cells 20567-1-AP

IP Result of anti-DICER1 (IP:20567-1-AP, 5ug; Detection:20567-1-AP 1:300) with Jurkat cells lysate 2000ug.

DICER1 Antibody IHC human testis tissue 20567-1-AP

Immunohistochemistry of paraffin-embedded human testis tissue slide using 20567-1-AP( DICER1 Antibody) at dilution of 1:200 (under 40x lens).

DICER1 Antibody IF HepG2 cells 20567-1-AP

Immunofluorescent analysis of HepG2 cells using 20567-1-AP(DICER1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human
Positive WB detected in:
HeLa cells, K-562 cells, HepG2 cells
Positive IP detected in:
Jurkat cells
Positive IHC detected in:
human testis tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
dicer 1, ribonuclease type III
Calculated molecular weight:
219 kDa
Observed molecular weight:
220-250 kDa,90 kDa
GenBank accession number:
NM_030621
Gene ID (NCBI):
23405
Gene symbol
DICER1
Synonyms
DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928

Background

DICER1, also named as DICER, HERNA and KIAA0928, belongs to the helicase family and Dicer subfamily. It is required for formation of the RNA induced silencing complex (RISC). DICER1 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. DICER1 cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs. The antibody is specific to DICER1. The calcualted molecular weight of DICER1 is 219 kDa, but modified DICER1 is about 220-250 kDa. DICER1 exists some isoforms with molecular weight 219 kDa and 93 kDa.


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