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DNAJC10 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 13101-1-AP

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Con: 42 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

DNAJC10 Antibody WB HeLa cells 13101-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 13101-1-AP(DNAJC10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

DNAJC10 Antibody IHC human pancreas cancer tissue 13101-1-AP

Immunohistochemical of paraffin-embedded human pancreas cancer using 13101-1-AP(DNAJC10 antibody) at dilution of 1:100 (under 10x lens)

DNAJC10 Antibody IF HeLa cells 13101-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 13101-1-AP(DNAJC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, mouse liver tissue
Positive IHC detected in:
human pancreas cancer tissue, human stomach tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:3000
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DNAJC10 fusion protein Ag3737
Full name:
DnaJ (Hsp40) homolog, subfamily C, member 10
Calculated molecular weight:
793aa,91 kDa
Observed molecular weight:
75-80 kDa
GenBank accession number:
BC034713
Gene ID (NCBI):
54431
Gene symbol
DNAJC10
Synonyms
DNAJC10, ER resident protein ERdj5, ERdj5, JPDI, Macrothioredoxin, MTHr

Background

DNAJC10(DnaJ homolog subfamily C member 10)is also named as ERDJ5. The human ER-resident protein (ERdj5) ubiquitously expresses and is abundant in secretory tissues such as pancreas and testis and it may be involved in assisting protein folding and quality control in the ER(PMID:12411443). The deduced 793-amino acid protein has a calculated molecular mass of about 91 kD. ERDJ5 contains an N-terminal hydrophobic sequence, followed by a type III DnaJ domain, 4 thioredoxin-like domains, and a C-terminal KDEL endoplasmic reticulum (ER) retention signal. It can be modified by N-linked glycosylation(PMID:12446677). The ERdj5 antibody displays a higher affinity for endogenous ERdj5u.


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