DOK3 Polyclonal antibody
DOK3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15749-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
15749-1-AP targets DOK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DOK3 fusion protein Ag8221 |
| Full Name | docking protein 3 |
| Calculated Molecular Weight | 496aa,53 kDa; 330aa,36 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC004564 |
| Gene Symbol | DOK3 |
| Gene ID (NCBI) | 79930 |
| RRID | AB_2095099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The adaptor proteins downstream of kinase 1 (DOK1) to DOK3 are mainly found in immune cells, and they are closely related, with some functional redundancy, although each DOK protein has distinct properties and functions. DOK1 to DOK3 inhibit LPS responses. DOK proteins have an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and four tyrosine-containing motifs that can bind to Src homology 2 (SH2) domains when phosphorylated. DOK3 was also shown to have an inhibitory role as it restricts Ca2+ and JNK activation in B cell receptor signaling. The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DOK3 antibody 15749-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Elevated expression of DOK3 indicates high suppressive immune cell infiltration and unfavorable prognosis of gliomas. |
