DOK3 Polyclonal antibody

DOK3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15749-1-AP

Synonyms

docking protein 3, DOK3, DOKL



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

15749-1-AP targets DOK3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOK3 fusion protein Ag8221
Full Name docking protein 3
Calculated molecular weight496aa,53 kDa; 330aa,36 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC004564
Gene symbol DOK3
Gene ID (NCBI) 79930
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The adaptor proteins downstream of kinase 1 (DOK1) to DOK3 are mainly found in immune cells, and they are closely related, with some functional redundancy, although each DOK protein has distinct properties and functions. DOK1 to DOK3 inhibit LPS responses. DOK proteins have an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and four tyrosine-containing motifs that can bind to Src homology 2 (SH2) domains when phosphorylated. DOK3 was also shown to have an inhibitory role as it restricts Ca2+ and JNK activation in B cell receptor signaling. The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.

Protocols

Product Specific Protocols
WB protocol for DOK3 antibody 15749-1-APDownload protocol
IHC protocol for DOK3 antibody 15749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

Elevated expression of DOK3 indicates high suppressive immune cell infiltration and unfavorable prognosis of gliomas.

Authors - Xiu Liu