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DOK4 Polyclonal antibody

DOK4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10481-2-AP

Synonyms

docking protein 4, DOK4, IRS 5, IRS5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DOK4 Polyclonal antibody (10481-2-AP)

    WB analysis using 10481-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10481-2-AP (DOK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10481-2-AP targets DOK4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DOK4 fusion protein Ag0734
Full Name docking protein 4
Calculated Molecular Weight 37 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC003541
Gene Symbol DOK4
Gene ID (NCBI) 55715
RRIDAB_2246205
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The INS-receptor substrate family plays important roles in cellular growth, signaling and survival. IRS5/DOK4 is a new member of this family identified, which is known as docking protein 4, or downstream of tyrosine kinase-4. DOK4 might have roles in INS and IGF-1 signaling as well. Expression of IRS5/DOK4 is regulated epigenetically via histone acetylating at its promoter. DOK4 is required at early stages in the myelination process, including the initial interaction with the axon, and is also involved in Schwann cell migration and proliferation, it also regulates GDNF-mediated neurite outgrowth during neuronal development through activation of the Rap1-ERK1/2 pathway. DOK4 may represent a novel negative regulator of T cells as other DOK family members like DOK1 and DOK2.

Protocols

Product Specific Protocols
WB protocol for DOK4 antibody 10481-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols