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  • KD/KO Validated

DR4 Polyclonal antibody

DR4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 24063-1-AP

Synonyms

APO2, CD261, Death receptor 4, DR4, TNFRSF10A, TRAIL R1, TRAIL receptor 1, TRAILR 1, TRAILR1



Tested Applications

Positive WB detected inJurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24063-1-AP is DR4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DR4 fusion protein Ag20927
Full Name tumor necrosis factor receptor superfamily, member 10a
Calculated molecular weight 50 kDa
Observed molecular weight 44-50 kDa
GenBank accession numberBC012866
Gene symbol TNFRSF10A
Gene ID (NCBI) 8797
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily. TRAIL activates apoptosis through the death receptors DR4 (also known as TRAILR1 and TNFRSF10A) and DR5 (also known as TRAILR2, KILLER and TNFRSF10B). DR4 and DR5 are single-pass type I membrane proteins that contain intracellular death domains (DD) and upon activation mediate apoptotic signals (PMID: 11163110). Binding of TRAIL to DR4 or DR5 results in receptor oligomerization and recruitment of FAS-associated protein with death domain (FADD) and caspase 8 to form a functional death-inducing signalling complex (DISC). Upon DISC formation, caspase 8 is cleaved and activated, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PMID: 18813321). DR4 or DR5 promotes the activation of NF-kappa-B and play an important role in inflammation (PMID: 9430227, 19434100).

Protocols

Product Specific Protocols
WB protocol for DR4 antibody 24063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,FC

Oncol Lett

Jinfukang induces cellular apoptosis through activation of Fas and DR4 in A549 cells.

Authors - Jun Lu
  • KD Validated
humanWB

Arch Biochem Biophys

Luteolin enhances TRAIL sensitivity in non-small cell lung cancer cells through increasing DR5 expression and Drp1-mediated mitochondrial fission.

Authors - Bin Wu
  • KD Validated
humanWB

Mar Drugs

Fucoidan Induces Apoptosis of HT-29 Cells via the Activation of DR4 and Mitochondrial Pathway.

Authors - Xu Bai
humanWB

Oncogenesis

JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells.

Authors - Q Wang
humanWB

Am J Cancer Res

PMID: 31598394

Authors - Ran An
humanWB

Cell Death Dis

Celastrol induces apoptosis and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells: an in vitro and in vivo study.

Authors - H-Y Li