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DTL Polyclonal antibody

DTL Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12896-1-AP

Synonyms

CDT2, CDW1, DCAF2, Denticleless protein homolog, DTL, L2DTL, RAMP

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DTL Polyclonal antibody (12896-1-AP)

    WB analysis of HeLa using 12896-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using DTL Polyclonal antibody (12896-1-AP)

    WB analysis of MCF-7 using 12896-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using DTL Polyclonal antibody (12896-1-AP)

    WB analysis of L02 using 12896-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12896-1-AP (DTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DTL Polyclonal antibody (12896-1-AP)

    IP experiment of mouse testis using 12896-1-AP

    IP result of anti-DTL (IP:12896-1-AP, 3ug; Detection:12896-1-AP 1:500) with mouse testis tissue lysate 8000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DTL Polyclonal antibody (12896-1-AP)

    IF Staining of HeLa using 12896-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12896-1-AP (DTL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, L02 cells, MCF-7 cells
Positive IP detected inmouse testis tissue
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12896-1-AP targets DTL in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DTL fusion protein Ag3589
Full Name denticleless homolog (Drosophila)
Calculated Molecular Weight 730 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC033540
Gene Symbol DTL
Gene ID (NCBI) 51514
RRIDAB_10639514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDT2, also named as L2DTL or RAMP, is one of the substrate receptors of the Cullin Ring Ubiquitin Ligase 4 that targets for ubiquitin mediated degradation a number of substrates, such as CDT1, p21 and CHK1, involved in the regulation of cell cycle and survival. CDT2 overexpression was reported in breast (PMID: 18542055), gastric (PMID: 19672268) and ovarian carcinomas (PMID: 23995842) and rhabdomyosarcomas (PMID: 19235922) and associated with the aggressiveness of hepatocellular carcinomas (PMID: 17106265).

Protocols

Product Specific Protocols
WB protocol for DTL antibody 12896-1-APDownload protocol
IF protocol for DTL antibody 12896-1-APDownload protocol
IP protocol for DTL antibody 12896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Adv

Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.

Authors - Chunxia Zhang
View Article
humanWB

Proteomics

Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs

Authors - Chengli Yu
View Article