Dysbindin Polyclonal antibody
Dysbindin Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11132-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, human testis tissue, mouse testis tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
11132-1-AP targets Dysbindin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Dysbindin fusion protein Ag1613 |
| Full Name | dystrobrevin binding protein 1 |
| Calculated Molecular Weight | 351 aa, 39 kDa |
| Observed Molecular Weight | 48 kDa, 36 kDa |
| GenBank Accession Number | BC011912 |
| Gene Symbol | DTNBP1 |
| Gene ID (NCBI) | 84062 |
| RRID | AB_11182503 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dysbindin, encoded by DTNBP1 (dystrobrevin binding protein 1), is a synaptic protein which is expressed in diverse neuronal populations throughout the brain. Genetic variation in DTNBP1 is associated with schizophrenia. Reduced expression of dysbindin has been reported in the dorsolateral prefrontal cortex (DLPFC) of schizophrenia cases. Several isoforms of dysbindin exist: 48-50 kDa of dysbindin-1A, 35-38 kDa of dysbindin-1B, and 30-32 kDa of dysbindin-1C. This antibody recognizes all of isoforms of dysbindin. (19617633)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Dysbindin antibody 11132-1-AP | Download protocol |
| IHC protocol for Dysbindin antibody 11132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol BLOC-1 is required for selective membrane protein trafficking from endosomes to primary cilia. | ||
Am J Respir Cell Mol Biol Gene-edited MLE-15 Cells as a Model for the Hermansky Pudlak Syndromes. | ||
Neuropharmacology Mice with dopaminergic neuron-specific deletion of DTNBP-1 gene show blunted nucleus accumbens dopamine release and associated behaviors. | ||
Neuroscience Protein kinase B/Akt1 phosphorylates dysbindin-1A at serine 10 to regulate neuronal development. | ||
Br J Pharmacol Dopaminergic signaling and behavioral alterations by Comt-Dtnbp1 genetic interaction and their clinical relevance | ||
Transl Psychiatry Deletion of Dtnbp1 in mice impairs threat memory consolidation and is associated with enhanced inhibitory drive in the amygdala. |
