Filter: AllWBIP

WB analysis of Y79 using 12334-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12334-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12334-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12334-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12334-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 12334-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12334-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.
IP experiment of mouse liver using 12334-1-AP
IP result of anti-E2F3 (IP:12334-1-AP, 4ug; Detection:12334-1-AP 1:500) with mouse liver tissue lysate 4000ug.

E2F3 Polyclonal antibody

Name: E2F3 Polyclonal antibody
Brand: Proteintech
SKU: 12334-1-AP

E2F3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 12334-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

E2F3, Q96AR0

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Y79 cells, HEK-293 cells, HepG2 cells, mouse liver tissue
Positive IP detected in	mouse liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below

Product Information

12334-1-AP targets E2F3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E2F3 fusion protein Ag3039 Predict reactive species
Full Name	E2F transcription factor 3
Calculated Molecular Weight	133 aa, 15 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC016847
Gene Symbol	E2F3
Gene ID (NCBI)	1871
RRID	AB_10597554
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00716
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E2F transcription factors regulate gene expression in concert with the retinoblastoma tumor suppressor family. These transcriptional complexes are master regulators of cell cycle progression, control the expression of genes involved in DNA repair, G2/M checkpoint and differentiation [PMID:22580460]. E2f3 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner [PMID:22960857].

Protocols

Product Specific Protocols
WB protocol for E2F3 antibody 12334-1-AP	Download protocol
IP protocol for E2F3 antibody 12334-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB, IHC	Nat Commun POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation. Authors - Boshi Wang View Article
human	WB	Mol Med Rep [Corrigendum] Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells. Authors - Dacai Xu View Article
human	WB	Mol Med Rep Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells. Authors - Dacai Xu View Article
human,mouse	WB	Cancer Lett PA28γ coordinates the cross-talk between cancer-associated fibroblasts and tumor cells to promote OSCC progression via HDAC1/E2F3/IGF2 signaling Authors - Zaiye Li View Article