• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

E2F3 Polyclonal antibody

E2F3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12334-1-AP

Synonyms

E2F3, Q96AR0

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Y79 cells using E2F3 Polyclonal antibody (12334-1-AP)

    WB analysis of Y79 using 12334-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using E2F3 Polyclonal antibody (12334-1-AP)

    WB analysis of HEK-293 using 12334-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using E2F3 Polyclonal antibody (12334-1-AP)

    WB analysis of HepG2 using 12334-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse liver tissue using E2F3 Polyclonal antibody (12334-1-AP)

    WB analysis of mouse liver using 12334-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using E2F3 Polyclonal antibody (12334-1-AP)

    WB analysis of HepG2 using 12334-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using E2F3 Polyclonal antibody (12334-1-AP)

    WB analysis of Y79 using 12334-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using E2F3 Polyclonal antibody (12334-1-AP)

    WB analysis of Y79 using 12334-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using E2F3 Polyclonal antibody (12334-1-AP)

    IP experiment of mouse liver using 12334-1-AP

    IP result of anti-E2F3 (IP:12334-1-AP, 4ug; Detection:12334-1-AP 1:500) with mouse liver tissue lysate 4000ug.

Tested Applications

Positive WB detected inY79 cells, HEK-293 cells, HepG2 cells, mouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12334-1-AP targets E2F3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F3 fusion protein Ag3039
Full Name E2F transcription factor 3
Calculated Molecular Weight 133 aa, 15 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC016847
Gene Symbol E2F3
Gene ID (NCBI) 1871
RRIDAB_10597554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E2F transcription factors regulate gene expression in concert with the retinoblastoma tumor suppressor family. These transcriptional complexes are master regulators of cell cycle progression, control the expression of genes involved in DNA repair, G2/M checkpoint and differentiation [PMID:22580460]. E2f3 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner [PMID:22960857].

Protocols

Product Specific Protocols
WB protocol for E2F3 antibody 12334-1-APDownload protocol
IP protocol for E2F3 antibody 12334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IHC

Nat Commun

POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.

Authors - Boshi Wang
View Article
humanWB

Mol Med Rep

[Corrigendum] Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.

Authors - Dacai Xu
View Article
humanWB

Mol Med Rep

Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.

Authors - Dacai Xu
View Article