HEK-293 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP( E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP( E2F3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night

HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP( E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 12334-1-AP( E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP( E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP( E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP( E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

IP Result of anti-E2F3 (IP:12334-1-AP, 4ug; Detection:12334-1-AP 1:500) with mouse liver tissue lysate 4000ug.