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EED Polyclonal antibody

EED Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16818-1-AP

Synonyms

EED, embryonic ectoderm development, HEED, Polycomb protein EED, WAIT 1, WAIT1

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using EED Polyclonal antibody (16818-1-AP)

    WB analysis of COLO 320 using 16818-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using EED Polyclonal antibody (16818-1-AP)

    WB analysis of PC-3 using 16818-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EED Polyclonal antibody (16818-1-AP)

    WB analysis of HeLa using 16818-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using EED Polyclonal antibody (16818-1-AP)

    WB analysis of K-562 using 16818-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using EED Polyclonal antibody (16818-1-AP)

    WB analysis of A431 using 16818-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using EED Polyclonal antibody (16818-1-AP)

    WB analysis of HepG2 using 16818-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using EED Polyclonal antibody (16818-1-AP)

    WB analysis of COLO 320 using 16818-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using EED Polyclonal antibody (16818-1-AP)

    IP experiment of PC-3 using 16818-1-AP

    IP result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EED Polyclonal antibody (16818-1-AP)

    IHC staining of human tonsillitis using 16818-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EED Polyclonal antibody (16818-1-AP)

    IHC staining of human tonsillitis using 16818-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP (EED Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EED Polyclonal antibody (16818-1-AP)

    IF Staining of HepG2 using 16818-1-AP

    Immunofluorescent analysis of (10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EED Polyclonal antibody (16818-1-AP)

    IF Staining of HeLa using 16818-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using EED Polyclonal antibody (16818-1-AP)

    FC experiment of HepG2 using 16818-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EED (16818-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inCOLO 320 cells, A431 cells, HeLa cells, HepG2 cells, K-562 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16818-1-AP targets EED in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EED fusion protein Ag10358
Full Name embryonic ectoderm development
Calculated Molecular Weight 466 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC068995
Gene Symbol EED
Gene ID (NCBI) 8726
RRIDAB_2262065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.

Protocols

Product Specific Protocols
WB protocol for EED antibody 16818-1-APDownload protocol
IHC protocol for EED antibody 16818-1-APDownload protocol
IF protocol for EED antibody 16818-1-APDownload protocol
IP protocol for EED antibody 16818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

A covalently bound inhibitor triggers EZH2 degradation through CHIP-mediated ubiquitination.

Authors - Xu Wang
View Article
humanWB

Eur J Med Chem

Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.

Authors - Cheng Wang
View Article

J Cell Sci

EZH1/SUZ12 complex positively regulates the transcription of NF-κB target genes via interaction with UXT.

Authors - Shuai-Kun Su
View Article
humanWB

Sci Rep

Polycomb repressive complex 2 facilitates the nuclear export of the influenza viral genome through the interaction with M1.

Authors - Masamitsu N Asaka
View Article
humanWB

Cell Signal

Mechanical force-sensitive lncRNA SNHG8 inhibits osteogenic differentiation by regulating EZH2 in hPDLSCs.

Authors - Zijie Zhang
View Article
humanWB

J Cancer

Transcriptional and H3K27ac related genome profiles in oral squamous cell carcinoma cells treated with metformin.

Authors - Shan Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Nikhil (Verified Customer) (01-07-2022)

HCT116 whole cell extract 20microg/lane. Lane 2-4 transient expression of EZH2.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HCT116
EED Antibody Western Blot validation (1:1000 dilution) in HCT116 (Cat no:16818-1-AP)