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PHD2 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 20368-1-AP

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Con: 59 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

PHD2 Antibody WB HEK-293 cells 20368-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP(PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

PHD2 Antibody IF HepG2 cells 20368-1-AP

Immunofluorescent analysis of HepG2 cells, using EGLN1 antibody 20368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PHD2 Antibody IHC human pancreas tissue 20368-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP( PHD2 antibody at dilution of 1:200 (under 10x lens).

PHD2 Antibody IHC human pancreas tissue 20368-1-AP

Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP( PHD2 antibody at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse
Positive WB detected in:
HEK-293 cells, HepG2 cells, mouse pancreas tissue
Positive IHC detected in:
human pancreas tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:100-1:400
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
egl nine homolog 1 (C. elegans)
Calculated molecular weight:
46 kDa
Observed molecular weight:
46 kDa,36 kDa
GenBank accession number:
NM_022051
Gene ID (NCBI):
54583
Gene symbol
EGLN1
Synonyms
C1orf12, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PNAS 137, SM 20, SM20, ZMYND6

Background

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.


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