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PHD2/EGLN1 Polyclonal antibody

PHD2/EGLN1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20368-1-AP

Synonyms

C1orf12, ECYT3, Egl nine homolog 1, EGLN1, HIF PH2, HIF prolyl hydroxylase 2, HIFPH2, HPH 2, HPH2, PHD2, PNAS 137, SM 20, SM20, ZMYND6

Validation Data Gallery

  • WB analysis of HEK-293 using 20368-1-AP

    WB analysis of HEK-293 using 20368-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20368-1-AP (PHD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20368-1-AP

    IF Staining of HepG2 using 20368-1-AP

    Immunofluorescent analysis of HepG2 cells, using EGLN1 antibody 20368-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human pancreas using 20368-1-AP

    IHC staining of human pancreas using 20368-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 20368-1-AP

    IHC staining of human pancreas using 20368-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20368-1-AP (PHD2 antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells, mouse pancreas tissue, HepG2 cells
Positive IHC detected inhuman pancreas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20368-1-AP targets PHD2/EGLN1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name egl nine homolog 1 (C. elegans)
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa, 36 kDa
GenBank accession numberNM_022051
Gene symbol EGLN1
Gene ID (NCBI) 54583
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EGLN1, also named as PHD2, SM-20, HPH-2 and HIF-PH2, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN1 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3). EGLN1 has 3 isoforms with MW of 46 kDa, 44 kDa and 36 kDa produced by alternative splicing. It mainly localizes in cytoplasm and can shuttle between the nucleus and cytoplasm (PubMed:19631610). The antibody is specific to EGLN1.

Protocols

Product Specific Protocols
WB protocol for PHD2/EGLN1 antibody 20368-1-APDownload protocol
IHC protocol for PHD2/EGLN1 antibody 20368-1-APDownload protocol
IF protocol for PHD2/EGLN1 antibody 20368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols