Tested Applications
| Positive WB detected in | A549 cells, mouse thymus tissue, mouse heart tissue, mouse lung tissue, HEK-293 cells, mouse liver tissue, HeLa cells, MCF-7 cells, HepG2 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue, human heart tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17504-1-AP targets EIF4A3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11701 Product name: Recombinant human EIF4A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-411 aa of BC003662 Sequence: MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4A, isoform 3 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC003662 |
| Gene Symbol | EIF4A3 |
| Gene ID (NCBI) | 9775 |
| RRID | AB_2097393 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P38919 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF4A3 antibody 17504-1-AP | Download protocol |
| IHC protocol for EIF4A3 antibody 17504-1-AP | Download protocol |
| IP protocol for EIF4A3 antibody 17504-1-AP | Download protocol |
| WB protocol for EIF4A3 antibody 17504-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-EIF4A3 antibody, catalog number 17504-1-AP, has 71 total citations. Publications appear in journals including Molecular Cancer, Cell Metabolism, Cell Research, Molecular Cell, Nucleic Acids Research, PNAS, Cell Death & Differentiation, Journal of Experimental & Clinical Cancer Research, Cell Reports, FASEB Journal, and RNA. Research fields span cancer biology (multiple tumor types), RNA biology (circular RNA, m6A modification, NMD), translation regulation, neurodegeneration, inflammation, virology, and stem cell biology.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation | ||
Mol Cancer EIF4A3/circPTGR1/miR-4725-5p positive- feedback loop promotes colorectal cancer progression via FAK/AKT signaling pathway. | ||
Mol Cancer Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer. | ||
Mol Cancer CircZFR/YTHDF3 axis drives lymph node metastasis in cervical cancer via FASN translation | ||
Mol Cancer Circular RNA circPHLPP2 promotes tumor growth and anti-PD-1 resistance through binding ILF3 to regulate IL36γ transcription in colorectal cancer. | ||
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. |
