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EIF4A3 Polyclonal antibody

Name: EIF4A3 Polyclonal antibody
Brand: Proteintech
SKU: 17504-1-AP

EIF4A3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 17504-1-AP

Synonyms

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of A549 using 17504-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 17504-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 17504-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 17504-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 17504-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 17504-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 17504-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17504-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17504-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17504-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 17504-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 17504-1-AP
IP result of anti-EIF4A3 (IP:17504-1-AP, 3ug; Detection:17504-1-AP 1:1000) with mouse heart tissue lysate 5000ug.
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of MCF-7 using 17504-1-AP
Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 17504-1-AP

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Tested Applications

Positive WB detected in	A549 cells, mouse thymus tissue, mouse heart tissue, mouse lung tissue, HEK-293 cells, mouse liver tissue, HeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human kidney tissue, human brain tissue, human heart tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 26 publications below
IHC	See 3 publications below
IF	See 10 publications below
IP	See 3 publications below
CoIP	See 3 publications below
RIP	See 8 publications below

Product Information

17504-1-AP targets EIF4A3 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	EIF4A3 fusion protein Ag11701
Full Name	eukaryotic translation initiation factor 4A, isoform 3
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	47 kDa
GenBank Accession Number	BC003662
Gene Symbol	EIF4A3
Gene ID (NCBI)	9775
RRID	AB_2097393
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

Protocols

Product Specific Protocols
WB protocol for EIF4A3 antibody 17504-1-AP	Download protocol
IHC protocol for EIF4A3 antibody 17504-1-AP	Download protocol
IF protocol for EIF4A3 antibody 17504-1-AP	Download protocol
IP protocol for EIF4A3 antibody 17504-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. Authors - Mi Kyung Park View Article
		Cell Res YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA. Authors - Hailing Shi View Article
human	IF,WB	Cell Death Differ eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy. Authors - Despoina Sakellariou KD Validated View Article
human	WB	Mol Cancer Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer. Authors - Huizhe Wu View Article
human	WB	J Nanobiotechnology Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling Authors - Xiao Liu View Article
human	WB,IF	Sci Total Environ A unique circ_0067716/EIF4A3 double-negative feedback loop impacts malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene Authors - Shuang Zhao View Article

EIF4A3 Polyclonal antibody

EIF4A3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of A549 using 17504-1-AP

WB analysis using 17504-1-AP

WB analysis of mouse thymus using 17504-1-AP

WB analysis of mouse thymus using 17504-1-AP

WB analysis of A549 using 17504-1-AP

WB analysis of mouse heart using 17504-1-AP

WB analysis of mouse lung using 17504-1-AP

WB analysis of HEK-293 using 17504-1-AP

WB analysis of mouse liver using 17504-1-AP

WB analysis of HeLa using 17504-1-AP

WB analysis of MCF-7 using 17504-1-AP

IP experiment of mouse heart using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IHC staining of human testis using 17504-1-AP

IHC staining of human testis using 17504-1-AP

IHC staining of human brain using 17504-1-AP

IHC staining of human brain using 17504-1-AP

IHC staining of human spleen using 17504-1-AP

IHC staining of human spleen using 17504-1-AP

IHC staining of human lung using 17504-1-AP

IHC staining of human lung using 17504-1-AP

IHC staining of human ovary using 17504-1-AP

IHC staining of human ovary using 17504-1-AP

IHC staining of human heart using 17504-1-AP

IHC staining of human heart using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IF Staining of MCF-7 using 17504-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA.

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.

Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling

A unique circ_0067716/EIF4A3 double-negative feedback loop impacts malignant transformation of human bronchial epithelial cells induced by benzo(a)pyrene