EIF6 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 10291-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 35 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
COLO 320 cells, A375 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue

Positive IHC detected in:
human prostate cancer tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
eukaryotic translation initiation factor 6

Calculated molecular weight:
27 kDa

Observed molecular weight:
27 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
EIF6

Synonyms
2, b, b(2)gcn, B(2)GCN homolog, CAB, eIF 6, EIF3A, EIF6, gcn, integrin beta 4 binding protein, ITGB4BP, p27(BBP), p27BBP
Background

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), It functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.


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