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EIF6 Polyclonal antibody

EIF6 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10291-1-AP

Synonyms

b(2)gcn, B(2)GCN homolog, CAB, eIF 6, EIF3A, EIF6, gcn, integrin beta 4 binding protein, ITGB4BP, p27(BBP), p27BBP



Tested Applications

Positive WB detected inCOLO 320 cells, A375 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10291-1-AP targets EIF6 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF6 fusion protein Ag0324
Full Name eukaryotic translation initiation factor 6
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001119
Gene symbol EIF6
Gene ID (NCBI) 3692
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

p27(BBP/eIF6) is an evolutionarily conserved protein that was originally identified as p27(BBP), It functions as an interactor of the cytoplasmic domain of integrin 4 and as the putative translation initiation factor eIF6. p27BBP is found in two pools: one nuclear pool enriched in the perinucleolar region, and one cytoplasmic pool. p27BBP binds to the fibronectin type III domains of integrin 4 subunit (ITGB4), an important functional component of hemidesmosomes, and help link ITGB4 to the intermediate filament cytoskeleton. In vitro and in vivo studies demonstrated that p27BBP is essential for cell viability and has a primary function in the biogenesis of the 60S ribosomal subunit. p27BBP protein is increased in rapidly cycling cells and decreased in villous cells committed to apoptotic cell death. In dysplastic colorectal adenomas and carcinomas, p27BBP displayed a large increase of its nucleolar component and was associated with the nuclear matrix. In particular, p27BBP increased progressively from adenomas to carcinomas and was related to the tumor stage.

Protocols

Product Specific Protocols
WB protocol for EIF6 antibody 10291-1-APDownload protocol
IHC protocol for EIF6 antibody 10291-1-APDownload protocol
IF protocol for EIF6 antibody 10291-1-APDownload protocol
FC protocol for EIF6 antibody 10291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Evid Based Complement Alternat Med

Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins.

Authors - Meina Shi
humanWB

Pediatr Blood Cancer

Impaired growth, hematopoietic colony formation, and ribosome maturation in human cells depleted of Shwachman-Diamond syndrome protein SBDS.

Authors - Sezgin Gulay G
humanWB

Biochem Biophys Res Commun

Mitochondrial function is impaired in yeast and human cellular models of Shwachman Diamond syndrome.

Authors - Henson Adrianna L AL
humanWB

Nature

MicroRNA silencing through RISC recruitment of eIF6.

Authors - Chendrimada Thimmaiah P TP
  • KD Validated