HuA/B/C/D Polyclonal antibody
HuA/B/C/D Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13032-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human cerebellum tissue, mouse cerebellum tissue, PC-13 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
13032-1-AP targets HuA/B/C/D in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HuA/B/C/D fusion protein Ag3678 |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
| Calculated Molecular Weight | 380 aa, 42 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC036071 |
| Gene Symbol | ELAVL4 |
| Gene ID (NCBI) | 1996 |
| RRID | AB_2096385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) is a human homologue of the Drosophilia gene ELAV, which is implicated in neuronal differentiation and maintenance. The ELAVL proteins are mRNA binding proteins and all members of the family contain three RNA-recognition motifs [PMID:12402251]. ELAVL-proteins also interact with inflammation-associated factors. The protein may be implicated in neurodegeneration through these complex mechanisms [PMID:15827745].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| IHC protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| IF protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| IP protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Neurosci Methods Efficient infection of organotypic hippocampal slice cultures with adenovirus carrying the transgene REST/NRSF. | ||
J Cosmet Dermatol Study on the correlation of high expression level of HuD with seborrheic keratosis and skin aging | ||
Med Mol Morphol Altered microbiota caused by disordered gut motility leads to an overactivation of intestinal immune system in APC1638T mice | ||
Biomed Pharmacother Shouhui Tongbian Capsule ameliorates constipation via gut microbiota-5-HT-intestinal motility axis |
