HuD-specific Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 24992-1-AP

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Con: 47 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
mouse brain tissue, mouse brain tissue, rat brain tissue

Positive IP detected in:
mouse brain tissue

Positive IHC detected in:
human brain tissue

Positive IF detected in:
SH-SY5Y cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)

Calculated molecular weight:
380aa,42 kDa

Observed molecular weight:
40 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ELAVL4

Synonyms
ELAV like protein 4, ELAVL4, Hu antigen D, HUD, PNEM
Background

ELAVL4, also named as HuD, is a 380 amino acid protein, which belongs to the RRM elav family. ELAVL4 is expressed in brain and localizes in the nucleus. ELAVL4 may play a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )


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