HuD-specific Polyclonal antibody

HuD-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24992-1-AP

Synonyms

ELAV like protein 4, ELAVL4, Hu antigen D, HUD, HuD-specific, PNEM



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24992-1-AP targets HuD-specific in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HuD-specific fusion protein Ag21830
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
Calculated Molecular Weight 380 aa, 42 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC036071
Gene Symbol ELAVL4
Gene ID (NCBI) 1996
RRIDAB_2879835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ELAVL4, also named as HuD, is a 380 amino acid protein, which belongs to the RRM elav family. ELAVL4 may play a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

Protocols

Product Specific Protocols
WB protocol for HuD-specific antibody 24992-1-APDownload protocol
IHC protocol for HuD-specific antibody 24992-1-APDownload protocol
IF protocol for HuD-specific antibody 24992-1-APDownload protocol
IP protocol for HuD-specific antibody 24992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Acta Neuropathol

Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis.

Authors - Sandra Diaz-Garcia
humanWB

Prog Neurobiol

RNA-binding protein ELAVL4/HuD ameliorates Alzheimer's disease-related molecular changes in human iPSC-derived neurons.

Authors - Robert J van der Linden
humanWB,RIP

FASEB J

ELAVL4 promotes the tumorigenesis of small cell lung cancer by stabilizing LncRNA LYPLAL1-DT and enhancing profilin 2 activation

Authors - Shuxin Li