ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16443-1-AP

Synonyms

ERK, ERK 2, ERK1/2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, NIH/3T3 cells, A431 cells, mouse kidney tissue, HEK-293 cells, PC-3 cells, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16443-1-AP targets ERK1/2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity A. sphaerocephala Krasch, Astragalus membranaceus, chicks, human, mouse, pig, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERK1/2 fusion protein Ag9772
Full Name mitogen-activated protein kinase 1
Calculated molecular weight 360 aa, 41 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC017832
Gene symbol MAPK1
Gene ID (NCBI) 5594
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
WB protocol for ERK1/2 antibody 16443-1-APDownload protocol
IHC protocol for ERK1/2 antibody 16443-1-APDownload protocol
IF protocol for ERK1/2 antibody 16443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int Immunopharmacol

Quercetin reduces Streptococcus suis virulence by inhibiting suilysin activity and inflammation.

Authors - Gen Li
ratWB

J Mol Neurosci

Treatment with MQA, a Derivative of Caffeoylquinic Acid, Provides Neuroprotective Effects against Cerebral Ischemia Through Suppression of the p38 Pathway and Oxidative Stress in Rats.

Authors - Long Chen
mouseWB

J Cell Sci

Tmem30a Deficiency in endothelial cells impairs cell proliferation and angiogenesis.

Authors - Shanshan Zhang
humanWB

J Cell Biochem

Identification of UGP2 as a progression marker that promotes cell growth and motility in human glioma.

Authors - Chun Zeng
mouse

Biomed Pharmacother

Renoprotective effect of scutellarin on cisplatin-induced renal injury in mice: Impact on inflammation, apoptosis, and autophagy.

Authors - Chao-Yue Sun
mouseWB

J Agric Food Chem

Comparison of the Hepatoprotective Effects of the Three Main Stilbenes from Mulberry Twigs.

Authors - Ya-Nan Jia