A431 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

A431 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

PC-3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

rat brain tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP( ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

rat kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP( ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human kidney slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded human kidney slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of HeLa cells using 16443-1-AP(ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)