Name: ERK1/2 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee

ERK1/2 Antibody WB HeLa cells 16443-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ERK1/2 Antibody WB NIH/3T3 cells 16443-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ERK1/2 Antibody IHC human liver tissue 16443-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:200 (under 40x lens).

ERK1/2 Antibody IF HeLa cells 16443-1-AP

Immunofluorescent analysis of HeLa cells using 16443-1-AP(ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat, zebrafish, A. sphaerocephala Krasch, Astragalus membranaceus, chicks, pig, sheep

Positive WB detected in:

HeLa cells, mouse brain tissue, NIH/3T3 cells, A431 cells, mouse kidney tissue, HEK-293 cells, PC-3 cells, rat brain tissue, rat kidney tissue

Positive IHC detected in:

human liver tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:1000-1:4000

IHC : 1:50-1:500

IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ERK1/2 fusion protein Ag9772

Full name:

mitogen-activated protein kinase 1

Calculated molecular weight:

360aa,41 kDa

Observed molecular weight:

38-44 kDa

GenBank accession number:

BC017832

Gene ID (NCBI):

5594

Gene symbol

MAPK1

Synonyms

ERK, ERK 2, ERK1/2, ERK2, ERT1, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 1, MAPK 2, MAPK1, MAPK2, p40, p41, p41mapk, p42 MAPK, P42MAPK, PRKM1, PRKM2

Background

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-43 kDa.

Showing 1- [6] of [94] Publications

Author:		Wang Cai-Ping CP
Pubmed ID:		24099731
Journal:		Neurochem Int
Title:		Isoquercetin protects cortical neurons from oxygen-glucose deprivation-reperfusion induced injury via suppression of TLR4-NF-кB signal pathway.
Application:		WB
KD validated
Species:		rat

Author:		Su Tzu-Rong TR
Pubmed ID:		24129178
Journal:		Int J Mol Sci
Title:		Inhibition of Melanogenesis by Gallic Acid: Possible Involvement of the PI3K/Akt, MEK/ERK and Wnt/β-Catenin Signaling Pathways in B16F10 Cells.
Application:		WB

Species:		mouse

Author:		Lijing Zhao
Pubmed ID:		24600487
Journal:		Int J Clin Exp Med
Title:		mTOR inhibitor AZD8055 inhibits proliferation and induces apoptosis in laryngeal carcinoma.
Application:		WB

Species:		human

Author:		Cai-Ping Wang
Pubmed ID:		24687774
Journal:		J Neurosci Res
Title:		Mulberroside a protects against ischemic impairment in primary culture of rat cortical neurons after oxygen-glucose deprivation followed by reperfusion.
Application:		WB

Species:

Author:		Cai-Ping Wang
Pubmed ID:		24986222
Journal:		J Physiol Biochem
Title:		Neuroprotective effect of schizandrin A on oxygen and glucose deprivation/reperfusion-induced cell injury in primary culture of rat cortical neurons.
Application:		WB

Species:		rat

Author:		Xiu Shan
Pubmed ID:		25672851
Journal:		Int J Oncol
Title:		Ginsenoside Rg3-induced EGFR/MAPK pathway deactivation inhibits melanoma cell proliferation by decreasing FUT4/LeY expression.
Application:		WB

Species:

WB Figures

ERK1/2 Antibody WB A431 cells 16443-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

A431 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ERK1/2 Antibody WB HEK-293 cells 16443-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ERK1/2 Antibody WB mouse brain tissue 16443-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse brain tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ERK1/2 Antibody WB mouse kidney tissue 16443-1-AP

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 16443-1-AP (ERK1/2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ERK1/2 Antibody WB PC-3 cells 16443-1-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 16443-1-AP(ERK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

ERK1/2 Antibody WB rat brain tissue 16443-1-AP

rat brain tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP( ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ERK1/2 Antibody WB rat kidney tissue 16443-1-AP

rat kidney tissue were subjected to SDS PAGE followed by western blot with 16443-1-AP( ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IHC Figures

ERK1/2 Antibody IHC human kidney tissue 16443-1-AP

Immunohistochemistry of paraffin-embedded human kidney slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16443-1-AP( ERK1/2 Antibody) at dilution of 1:200 (under 40x lens).

IF Figures

Immunofluorescent analysis of HeLa cells using 16443-1-AP(ERK1/2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ERK1/2 Antibody 94 Publications

Rabbit Polyclonal| Catalog number: 16443-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [6] of [94] Publications

WB Figures

IHC Figures

IF Figures

ERK1/2 Antibody 94 Publications

Rabbit Polyclonal| Catalog number: 16443-1-AP

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Related Primary Antibodies

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background