Various lysates were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 21244-1-AP( ER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

MCF-7 cells were subjected to SDS PAGE followed by western blot with 21244-1-AP( ER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP( ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21244-1-AP( ER Antibody) at dilution of 1:300 incubated at 4 degree celsius over night

rat testis tissue were subjected to SDS PAGE followed by western blot with 21244-1-AP( ER Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21244-1-AP( ER antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21244-1-AP( ER antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21244-1-AP( ER Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 21244-1-AP( ER Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP( ER Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 21244-1-AP( ER Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 21244-1-AP( ER Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).