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  • KD/KO Validated

ER Polyclonal antibody

ER Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21244-1-AP

Synonyms

ER, ER alpha, Era, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21244-1-AP is ER Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity cow, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ER fusion protein Ag15738
Full Name estrogen receptor 1
Calculated molecular weight 595 aa, 66 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC128573
Gene symbol ESR1
Gene ID (NCBI) 2099
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Protocols

Product Specific Protocols
WB protocol for ER antibody 21244-1-APDownload protocol
IHC protocol for ER antibody 21244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Phytother Res

Silibinin protects rat pancreatic β-cell through up-regulation of estrogen receptors' signaling against amylin- or Aβ1-42 -induced reactive oxygen species/reactive nitrogen species generation.

Authors - Jing Yang
  • KD Validated
ratWB

Mol Med Rep

ERα and Wnt/β‑catenin signaling pathways are involved in angelicin‑dependent promotion of osteogenesis.

Authors - Luna Ge
WB

Mol Med Rep

Saikosaponin‑D improves fear memory deficits in ovariectomized rats via the action of estrogen receptor‑α in the hippocampus.

Authors - Lina Liu
ratWB,IHC

Biochem Cell Biol

Rutin attenuates cerebral ischemia/reperfusion injury in ovariectomized rats via estrogen receptor-mediated BDNF-TrkB and NGF-TrkA signaling.

Authors - Hong Liu
mouseWB

J Biomed Sci

Dioscin promotes osteoblastic proliferation and differentiation via Lrp5 and ER pathway in mouse and human osteoblast-like cell lines.

Authors - Chunfang Zhang
humanWB

J Oncol

TP53 Mutations Promote Immunogenic Activity in Breast Cancer.

Authors - Zhixian Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (05-07-2019)

There are multiple bands for this antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: MCF10A/MCF7
ER Antibody Western Blot, validation (1:1000 dilution) in MCF10A/MCF7 (Cat no:21244-1-AP)