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ER Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 21244-1-AP

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Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

ER Antibody WB HeLa cells 21244-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 21244-1-AP (ER antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ER Antibody IHC human breast cancer tissue 21244-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21244-1-AP( ER antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ER Antibody IHC human ovary tumor tissue 21244-1-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21244-1-AP( ER Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected in:
human breast cancer tissue, human cervical cancer tissue, human ovary tumor tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:200-1:800
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ER fusion protein Ag15738
Full name:
estrogen receptor 1
Calculated molecular weight:
595aa,66 kDa
Observed molecular weight:
65-70 kDa
GenBank accession number:
BC128573
Gene ID (NCBI):
2099
Gene symbol
ESR1
Synonyms
ER, ER alpha, Era, ESR, ESR1, ESRA, Estradiol receptor, Estrogen receptor, estrogen receptor 1, NR3A1

Background

The estrogen receptor (ESR, ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. ESR1, also known as ESR or NR3A1, belongs to the nuclear hormone receptor family and NR3 subfamily. It is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. ESR1 can activate the transcriptional activity of TFF1.[PMID: 11731608,10970861]

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ER Rabbit Polyclonal antibody

Wei (Verified Customer), 05-07-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
ER Antibody Western Blot, validation (1:1000 dilution) in MCF10A/MCF7 (Cat no:21244-1-AP)

ER Antibody Western Blot, validation (1:1000 dilution) in MCF10A/MCF7 (Cat no:21244-1-AP)

Cell/Tissue Type
MCF10A/MCF7
Lot Number
There are multiple bands for this antibody.
Proteintech Response
We are sorry to hear that this antibody performed poorly in your experiments. We will put this antibody through QC again and make a new batch if necessary.

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