EXOSC7 Polyclonal antibody

EXOSC7 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25292-1-AP

Synonyms

EAP1, EXOSC7, exosome component 7, hRrp42p, KIAA0116, p8, RRP42, Rrp42p



Tested Applications

Positive WB detected inMCF-7 cells, A431 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25292-1-AP is EXOSC7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOSC7 fusion protein Ag18212
Full Name exosome component 7
Calculated molecular weight 291 aa, 32 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC012831
Gene symbol EXOSC7
Gene ID (NCBI) 23016
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. EXOSC7 also named as RRP42 or EAP1 is a 291 amino acid protein, which belongs to the RNase PH family. EXOSC7 is one of the six RNase-PH domain subunits of the exosome and required for the processing of the 7S pre-RNA

Protocols

Product Specific Protocols
WB protocol for EXOSC7 antibody 25292-1-APDownload protocol
IHC protocol for EXOSC7 antibody 25292-1-APDownload protocol
IF protocol for EXOSC7 antibody 25292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols