F9 Antibody

F9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21481-1-AP

Synonyms

Christmas factor, coagulation factor IX, F9, FIX, HEMB, PTC



Tested Applications

Positive WB detected inTunicamycin treated HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The 21481-1-AP immunogen is Fusion Protein F9 protein expressed in E. coli.

Reactivity human
Cited Species mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen F9 fusion protein Ag15723
Full Name coagulation factor IX
Calculated molecular weight 461aa,52 kDa
Observed molecular weight 56 kDa
GenBank accession number BC109215
Gene symbol F9
Gene ID (NCBI) 2158
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond.

Protocols

Product Specific Protocols
WB protocol for F9 antibody 21481-1-APDownload protocol
IHC protocol for F9 antibody 21481-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO Mol Med

CRISPR/Cas9-mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse.

Authors - Yuting Guan