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FADD Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 14906-1-AP

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

FADD Antibody IHC human lung cancer tissue 14906-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 14906-1-AP(FADD antibody) at dilution of 1:50 (under 10x lens)

FADD Antibody IHC human lung cancer tissue 14906-1-AP

Immunohistochemical of paraffin-embedded human lung cancer using 14906-1-AP(FADD antibody) at dilution of 1:50 (under 40x lens)

FADD Antibody WB HT-1080 cells 14906-1-AP

HT-1080 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP( FADD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

FADD Antibody IP A549 cells 14906-1-AP

IP Result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug.

FADD Antibody IF HT-1080 cells 14906-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HT-1080 cells using 14906-1-AP(FADD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, monkey, sheep
Positive WB detected in:
HT-1080 cells, mouse pancreas tissue
Positive IP detected in:
A549 cells
Positive IHC detected in:
human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HT-1080 cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FADD fusion protein Ag6701
Full name:
Fas (TNFRSF6)-associated via death domain
Calculated molecular weight:
23 kDa
Observed molecular weight:
23-30 kDa
GenBank accession number:
BC000334
Gene ID (NCBI):
8772
Gene symbol
FADD
Synonyms
FADD, GIG3, MORT1, Protein FADD

Background

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)


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