Filter: AllWBIPIHCIF/ICC

WB analysis using 14906-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 14906-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14906-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14906-1-AP
mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 14906-1-AP
IP result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug.
IHC staining of mouse kidney using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14906-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (14906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

FADD Polyclonal antibody

Name: FADD Polyclonal antibody
Brand: Proteintech
SKU: 14906-1-AP
Rating: 5.00 (1 reviews)

FADD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14906-1-AP

Publications(51)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (51)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HT-1080 cells, mouse pancreas tissue, A549 cells, mouse bone marrow, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected in	A549 cells
Positive IHC detected in	human lung cancer tissue, human colon tissue, human cervical cancer tissue, rat kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 46 publications below
IHC	See 7 publications below
IP	See 5 publications below
CoIP	See 1 publications below

Product Information

14906-1-AP targets FADD in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, monkey, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FADD fusion protein Ag6701 Predict reactive species
Full Name	Fas (TNFRSF6)-associated via death domain
Calculated Molecular Weight	23 kDa
Observed Molecular Weight	23-30 kDa
GenBank Accession Number	BC000334
Gene Symbol	FADD
Gene ID (NCBI)	8772
RRID	AB_2100486
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13158
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

Protocols

Product Specific Protocols
WB protocol for FADD antibody 14906-1-AP	Download protocol
IHC protocol for FADD antibody 14906-1-AP	Download protocol
IF protocol for FADD antibody 14906-1-AP	Download protocol
IP protocol for FADD antibody 14906-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. Authors - Jia-Yuan Zhang View Article
human,mouse	WB	Cell Commun Signal Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury Authors - Wenjing Sun View Article
mouse	WB	PLoS Biol A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5. Authors - Yingying Zhang View Article
human	WB	Eur J Med Chem PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism Authors - Hongyan Lin View Article
rat	WB,IHC	Cancers (Basel) Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1 Authors - Tiantian Qiu View Article
human	WB,IP	Int Immunopharmacol EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis Authors - Huiling Zhang View Article