|Positive WB detected in||K-562 cells, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, Hela cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13755-1-AP targets FMR1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||FMR1 fusion protein Ag4697|
|Full Name||fragile X mental retardation 1|
|Calculated molecular weight||632 aa, 71 kDa|
|Observed molecular weight||59-72 kDa|
|GenBank accession number||BC038998|
|Gene ID (NCBI)||2332|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.
|Product Specific Protocols|
|WB protocol for FMR1 antibody 13755-1-AP||Download protocol|
|IHC protocol for FMR1 antibody 13755-1-AP||Download protocol|
|IF protocol for FMR1 antibody 13755-1-AP||Download protocol|
|IP protocol for FMR1 antibody 13755-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marta (Verified Customer) (02-24-2020)
not specific enough in WB. Not tested in IP worst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.