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FMR1 Polyclonal antibody

Name: FMR1 Polyclonal antibody
Brand: Proteintech
SKU: 13755-1-AP
Rating: 3.00 (2 reviews)

FMR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Reviews (2)

Cat no : 13755-1-AP

Synonyms

FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1

Validation Data Gallery

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WB analysis of Jurkat cells using 13755-1-AP
WB result of FMR1 antibody (13755-1-AP, 1:1500) with si-Control and si-FMR1 transfected Jurkat cells. FMR1 gene has many isoforms with MW 59-72 kDa.
WB analysis of K-562 using 13755-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13755-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 13755-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13755-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13755-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 13755-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13755-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13755-1-AP (FMRP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 13755-1-AP
IP result of anti-FMR1 (IP:13755-1-AP, 4ug; Detection:13755-1-AP 1:1000) with HeLa cells lysate 2800ug.
IP experiment of HeLa cells using 13755-1-AP
IP result of anti-FMRP (13755-1-AP for IP and Detection).
IHC staining of human colon using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 13755-1-AP (FMRP antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human colon cancer using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 13755-1-AP (FMRP antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 13755-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13755-1-AP (FMR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 13755-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13755-1-AP
Immunofluorescent analysis of Hela cells, using FMR1 antibody 13755-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IF Staining of HepG2 using 13755-1-AP
Immunofluorescent analysis of HepG2 cells using 13755-1-AP (FMRP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
FC experiment of Jurkat using 13755-1-AP
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human FMR1 (13755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 13755-1-AP

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Tested Applications

Positive WB detected in	K-562 cells, mouse brain tissue, mouse thymus tissue, Jurkat cells, HeLa cells, HEK-293 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon tissue, human liver tissue, human colon cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells, Hela cells
Positive FC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 2 publications below

Product Information

13755-1-AP targets FMR1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FMR1 fusion protein Ag4697
Full Name	fragile X mental retardation 1
Calculated Molecular Weight	632 aa, 71 kDa
Observed Molecular Weight	59-72 kDa
GenBank Accession Number	BC038998
Gene Symbol	FMR1
Gene ID (NCBI)	2332
RRID	AB_2262872
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Protocols

Product Specific Protocols
WB protocol for FMR1 antibody 13755-1-AP	Download protocol
IHC protocol for FMR1 antibody 13755-1-AP	Download protocol
IF protocol for FMR1 antibody 13755-1-AP	Download protocol
IP protocol for FMR1 antibody 13755-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. Authors - David W Sanders View Article
human	WB	Cancer Cell Int MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome. Authors - Yu Men View Article
human	WB	Biology (Basel) In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome. Authors - Olga Gourdomichali View Article
human	WB,IHC	Cell Death Dis FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner Authors - Yuhan Hu KD Validated View Article
human	WB	FASEB J Decreased DANCR contributes to high glucose-induced extracellular matrix accumulation in human renal mesangial cell via regulating the TGF-β/Smad signaling Authors - Feng Liu View Article
human	WB	Dev Cell Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome Authors - Ji Geng View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Parisa (Verified Customer) (09-26-2023)	We used for WB, nor high specificity as we could see different bands (they could be different isoforms of the protein). But it was sensitive and we could see the band pulled down in IP for our protein of interest. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Hek293
FH Marta (Verified Customer) (02-24-2020)	not specific enough in WB. Not tested in IPworst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL. Applications: Western Blot, Primary Antibody Dilution: 1/1000 Cell Tissue Type: Brain mouse and rat FMR1 Antibody Western Blot, validation (1/1000 dilution) in Brain mouse and rat (Cat no:13755-1-AP)

FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of Jurkat cells using 13755-1-AP

WB analysis of K-562 using 13755-1-AP

WB analysis of mouse brain using 13755-1-AP

WB analysis of mouse thymus using 13755-1-AP

WB analysis of HeLa using 13755-1-AP

WB analysis of HEK-293 using 13755-1-AP

WB analysis of K-562 using 13755-1-AP

WB analysis of Jurkat using 13755-1-AP

IP experiment of HeLa using 13755-1-AP

IP experiment of HeLa cells using 13755-1-AP

IHC staining of human colon using 13755-1-AP

IHC staining of human colon using 13755-1-AP

IHC staining of human gliomas using 13755-1-AP

IHC staining of human colon cancer using 13755-1-AP

IHC staining of human gliomas using 13755-1-AP

IHC staining of human liver using 13755-1-AP

IHC staining of human colon cancer using 13755-1-AP

IHC staining of human liver using 13755-1-AP

IF Staining of HepG2 using 13755-1-AP

IF Staining of HeLa using 13755-1-AP

IF Staining of HepG2 using 13755-1-AP

FC experiment of Jurkat using 13755-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

MiR-323a-3p acts as a tumor suppressor by suppressing FMR1 and predicts better esophageal squamous cell carcinoma outcome.

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

FMR1 promotes the progression of colorectal cancer cell by stabilizing EGFR mRNA in an m6A-dependent manner

Decreased DANCR contributes to high glucose-induced extracellular matrix accumulation in human renal mesangial cell via regulating the TGF-β/Smad signaling

Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome

Reviews