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  • KD/KO Validated

FMR1 Polyclonal antibody

FMR1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13755-1-AP

Synonyms

FMR1, FMRP, fragile X mental retardation 1, FRAXA, POF, POF1, Protein FMR 1



Tested Applications

Positive WB detected inK-562 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13755-1-AP targets FMR1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMR1 fusion protein Ag4697
Full Name fragile X mental retardation 1
Calculated molecular weight 632 aa, 71 kDa
Observed molecular weight 59-72 kDa
GenBank accession numberBC038998
Gene symbol FMR1
Gene ID (NCBI) 2332
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The selective RNA-binding protein FMRP forms a messenger ribonucleoprotein complex that associates with polyribosomes, implicating in regulation of translation. FMR1 is a component of the CYFIP1-EIF4E-FMR1 complex which binds to and reperss the mRNA. It also has a role in the transport of mRNA from the nucleus to the cytoplasm. FMR1 exists several isforms and the molecular weight of FMR1 is about 59-72 kDa.

Protocols

Product Specific Protocols
WB protocol for FMR1 antibody 13755-1-APDownload protocol
IHC protocol for FMR1 antibody 13755-1-APDownload protocol
IF protocol for FMR1 antibody 13755-1-APDownload protocol
IP protocol for FMR1 antibody 13755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Cell

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization.

Authors - David W Sanders

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marta (Verified Customer) (02-24-2020)

not specific enough in WB. Not tested in IP worst than the antibody that we have available in the antibody. Need a long exposure with a high sensible ECL.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Brain mouse and rat
FMR1 Antibody Western Blot, validation (1/1000 dilution) in Brain mouse and rat (Cat no:13755-1-AP)