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  • Featured Product
  • KD/KO Validated

FOXC2 Polyclonal antibody

FOXC2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23066-1-AP

Synonyms

FKHL14, Forkhead box protein C2, FOXC2, LD, Mesenchyme fork head protein 1, MFH 1, MFH 1 protein, MFH1, Transcription factor FKH 14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXC2 Polyclonal antibody (23066-1-AP)

    WB analysis using 23066-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23066-1-AP (FOXC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using FOXC2 Polyclonal antibody (23066-1-AP)

    WB analysis of rat spleen using 23066-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 23066-1-AP (FOXC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using FOXC2 Polyclonal antibody (23066-1-AP)

    IF Staining of A375 using 23066-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 23066-1-AP (FOXC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHCT 116 cells, rat spleen tissue, MCF-7 cells
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23066-1-AP targets FOXC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXC2 fusion protein Ag19378
Full Name forkhead box C2 (MFH-1, mesenchyme forkhead 1)
Calculated Molecular Weight 501 aa, 54 kDa
Observed Molecular Weight 54-68 kDa
GenBank Accession NumberBC113437
Gene Symbol FOXC2
Gene ID (NCBI) 2303
RRIDAB_2879204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708) This antibody recognizes the 56 kDa FOXC2 protein, but the phosphorylated FOXC2 may be a 56-65 kDa protein. The 62kDa band was also detected in the study(PMID: 19540201).

Protocols

Product Specific Protocols
WB protocol for FOXC2 antibody 23066-1-APDownload protocol
IF protocol for FOXC2 antibody 23066-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Hypoxia-induced feedback of HIF-1α and lncRNA-CF129 contributes to pancreatic cancer progression through stabilization of p53 protein.

Authors - Mingliang Liu
  • KD Validated
View Article
mouseWB

Clin Transl Med

VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

Authors - Qiu-Yue Lin
View Article
humanWB,IF

Exp Cell Res

Long non-coding RNA GClnc1 knockdown suppresses progression of epithelial ovarian cancer by recruiting FOXC2 to disrupt the NOTCH1/NF-κB/Snail pathway.

Authors - Dandan Wu
View Article
ratWB

Am J Transl Res

Naringin promotes osteogenic differentiation of bone marrow stromal cells by up-regulating Foxc2 expression via the IHH signaling pathway.

Authors - Fei-Xiang Lin
View Article
WB

Acta Biochim Biophys Sin (Shanghai)

STK33 plays an important positive role in the development of human large cell lung cancers with variable metastatic potential.

Authors - Ping Wang
View Article
humanWB,IHC

Cell Death Dis

Decreased ZNF750 promotes angiogenesis in a paracrine manner via activating DANCR/miR-4707-3p/FOXC2 axis in esophageal squamous cell carcinoma.

Authors - Yanghui Bi
View Article