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- KD/KO Validated
FOXM1 Polyclonal antibody
FOXM1 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, chIP, ELISA
Conjugate
Unconjugated
Cat no : 13147-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse thymus tissue, L02 cells, mouse colon tissue, human testis tissue, mouse testis tissue, mouse spleen tissue, HeLa cells |
| Positive IP detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 25 publications below |
| IHC | See 7 publications below |
| IF | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
13147-1-AP targets FOXM1 in WB, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FOXM1 fusion protein Ag3729 |
| Full Name | forkhead box M 1 |
| Calculated molecular weight | 83 kDa |
| Observed molecular weight | 110 kDa |
| GenBank accession number | BC006192 |
| Gene symbol | FOXM1 |
| Gene ID (NCBI) | 2305 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Forkhead box M1 (FOXM1), also named HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1,FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| IP protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Silibinin-induced glioma cell apoptosis by PI3K-mediated but Akt-independent downregulation of FoxM1 expression. | ||
Biomed Res Int Integrated Bioinformatics Analysis of Master Regulators in Anaplastic Thyroid Carcinoma. | ||
Breast Cancer Res Dynamically decreased miR-671-5p expression is associated with oncogenic transformation and radiochemoresistance in breast cancer. | ||
Biomolecules Suppression of Hepatocellular Carcinoma Progression through FOXM1 and EMT Inhibition via Hydroxygenkwanin-Induced miR-320a Expression.
| ||
Oncotarget miR-671-5p inhibits epithelial-to-mesenchymal transition by downregulating FOXM1 expression in breast cancer. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Iram (Verified Customer) (09-17-2020) | FOXM1 antibody giving a very clear bands.
|
FH Terri (Verified Customer) (11-05-2019) | This antibody distinguishes multiple isoforms of FOXM1.
|
