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FOXM1 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 13147-1-AP

Featured Product KD/KO validated

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Con: 43 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

FOXM1 Antibody WB HeLa cells 13147-1-AP

WB result of FOXM1 antibody (13147-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXM1 transfected HeLa cells

FOXM1 Antibody WB mouse thymus tissue 13147-1-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13147-1-AP(FOXM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

FOXM1 Antibody IP L02 cells 13147-1-AP

IP Result of anti-FOXM1 (IP:13147-1-AP, 5ug; Detection:13147-1-AP 1:700) with L02 cells lysate 1560ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse thymus tissue, L02 cells, mouse colon tissue, human testis tissue, mouse testis tissue, mouse spleen tissue, HeLa cells
Positive IP detected in:
L02 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FOXM1 fusion protein Ag3729
Full name:
forkhead box M1
Calculated molecular weight:
83 kDa
Observed molecular weight:
110 kDa
GenBank accession number:
BC006192
Gene ID (NCBI):
2305
Gene symbol
FOXM1
Synonyms
FKHL16, forkhead box M1, Forkhead box protein M1, FOXM1, FOXM1B, HFH 11, HFH11, HNF 3, HNF 3/fork head homolog 11, INS 1, M phase phosphoprotein 2, MPHOSPH2, MPP 2, MPP2, PIG29, TGT3, Transcription factor Trident, TRIDENT, WIN

Background

Forkhead box M1 (FOXM1), also named HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1,FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa)


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