Tested Applications
| Positive WB detected in | HT-29 cells, HEK-293 cells, HeLa cells, human testis tissue, mouse colon tissue, mouse spleen tissue, mouse testis tissue, mouse thymus tissue, MCF-7 cells, L02 cells, A431 cells |
| Positive IP detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 15 publications below |
| WB | See 77 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 8 publications below |
| RIP | See 1 publications below |
Product Information
13147-1-AP targets FOXM1 in WB, IP, CoIP, chIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FOXM1 fusion protein Ag3729 Predict reactive species |
| Full Name | forkhead box M 1 |
| Calculated Molecular Weight | 83 kDa |
| Observed Molecular Weight | 84-110 kDa |
| GenBank Accession Number | BC006192 |
| Gene Symbol | FOXM1 |
| Gene ID (NCBI) | 2305 |
| RRID | AB_2106213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08050 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| IP protocol for FOXM1 antibody 13147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Discov Interferon-a potentiates anti-PD-1 efficacy by remodeling glucose metabolism in the hepatocellular carcinoma microenvironment.
| ||
Nat Commun K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis | ||
Acta Pharm Sin B Constitutive androstane receptor induced-hepatomegaly and liver regeneration is partially via yes-associated protein activation. | ||
Nat Commun Vagus-macrophage-hepatocyte link promotes post-injury liver regeneration and whole-body survival through hepatic FoxM1 activation. | ||
Nat Commun FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence. | ||
Nat Commun circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Jordan (Verified Customer) (11-07-2024) | Works well at 1:1000 overnight at 4c
|
FH Angie (Verified Customer) (08-27-2024) | A549 lysate was subjected to western blot with FOXM1 antibody used at 1:10000 and incubated at room temperature for 1.5 hours. Secondary antibody: Donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution incubated at room temperature for 1 hour. Image is shown in grayscale. Several bands were observed.
|
FH Kausik (Verified Customer) (02-21-2022) | The antibody worked well with immunoprecipitation and ChIP experiments and we had acceptable results with immunoblotting.
|
FH Iram (Verified Customer) (09-17-2020) | FOXM1 antibody giving a very clear bands.
|
FH Terri (Verified Customer) (11-05-2019) | This antibody distinguishes multiple isoforms of FOXM1.
|
