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FRK Polyclonal antibody

FRK Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16197-1-AP

Synonyms

FRK, fyn related kinase, GTK, Protein tyrosine kinase 5, PTK5, RAK, Tyrosine protein kinase FRK

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FRK Polyclonal antibody (16197-1-AP)

    WB analysis using 16197-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16197-1-AP (FRK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using FRK Polyclonal antibody (16197-1-AP)

    IHC staining of human urothelial carcinoma using 16197-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16197-1-AP (FRK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using FRK Polyclonal antibody (16197-1-AP)

    IHC staining of human urothelial carcinoma using 16197-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16197-1-AP (FRK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FRK Polyclonal antibody (16197-1-AP)

    IF Staining of HepG2 using 16197-1-AP

    Immunofluorescent analysis of HepG2 cells, using FRK antibody 16197-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using FRK Polyclonal antibody (16197-1-AP)

    FC experiment of HepG2 using 16197-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug FRK antibody (16197-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, NIH/3T3 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16197-1-AP is FRK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FRK fusion protein Ag10372
Full Name fyn-related kinase
Calculated Molecular Weight 505 aa, 56 kDa
Observed Molecular Weight 54-57 kDa
GenBank Accession NumberBC012916
Gene Symbol FRK
Gene ID (NCBI) 2444
RRIDAB_10639525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named as PTK5(protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).

Protocols

Product Specific Protocols
WB protocol for FRK antibody 16197-1-APDownload protocol
IHC protocol for FRK antibody 16197-1-APDownload protocol
IF protocol for FRK antibody 16197-1-APDownload protocol
FC protocol for FRK antibody 16197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols