Tested Applications
| Positive WB detected in | HepG2 cells, COLO 320 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18413-1-AP targets FURIN in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13261 Product name: Recombinant human FURIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 495-794 aa of BC012181 Sequence: YNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDTHYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPEVVAGLSCAFIVLVFVTVFLVLQLRSGFSFRGVKVYTMDRGLISYKGLPPEAWQEECPSDSEEDEGRGERTAFIKDQSAL Predict reactive species |
| Full Name | furin (paired basic amino acid cleaving enzyme) |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 90-110 kDa |
| GenBank Accession Number | BC012181 |
| Gene Symbol | FURIN |
| Gene ID (NCBI) | 5045 |
| RRID | AB_2878542 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09958 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The molecular weight of mature furin is 75-90 kDa and that of pre-pro furin is 95 kDa. With glycosylation and other post-translational modifications, the molecular weight of pre-pro furin will be migrated to 100-110 kDa. (PMID: 1644796, 1885622, 31336100, 35322530).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FURIN antibody 18413-1-AP | Download protocol |
| IHC protocol for FURIN antibody 18413-1-AP | Download protocol |
| IP protocol for FURIN antibody 18413-1-AP | Download protocol |
| WB protocol for FURIN antibody 18413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FURIN Polyclonal antibody (Cat# 18413-1-AP) has 32 citations across journals including Cell Research, Nature Aging, Nature Communications, Cell Reports, Oncogene, FASEB Journal, PLoS Pathogens, Journal of Virology, and eLife. Research fields span oncology (hepatocellular carcinoma, breast cancer, pancreatic cancer, lung adenocarcinoma), SARS-CoV-2/COVID-19 pathogenesis, epithelial-mesenchymal transition, ferroptosis, viral entry mechanisms, neurocognitive disorders, renal injury, and iron metabolism.
| Species | Application | Title |
|---|---|---|
Cell Res Famsin, a novel gut-secreted hormone, contributes to metabolic adaptations to fasting via binding to its receptor OLFR796 | ||
Nat Aging Brain-wide alterations revealed by spatial transcriptomics and proteomics in COVID-19 infection | ||
Nat Commun Secreted protease PRSS35 suppresses hepatocellular carcinoma by disabling CXCL2-mediated neutrophil extracellular traps
| ||
J Exp Clin Cancer Res Ficolin 3 promotes ferroptosis in HCC by downregulating IR/SREBP axis-mediated MUFA synthesis | ||
Adv Sci (Weinh) Furin Egress from the TGN is Regulated by Membrane-Associated RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32 (USP32) via Nondegradable K33-Polyubiquitination
| ||
Phytomedicine Tibetan medicine Liuwei Muxiang pills (LWMX pills) effectively protects mice from chronic non-atrophic gastritis |
Reviews
What customers say
Users report consistent performance of this Furin antibody across Western blot and immunofluorescence. In WB (1:1000), two bands around 80 and 104 kDa were detected in T-ALL cell lines, with one reviewer noting a strong expected band in MEFs. For IF (1:200, PFA fixation with saponin permeabilization), the antibody successfully labeled both cell surface-like and Golgi localization in 293T and HeLa cells. Overall ratings average 4.5 out of 5, with users finding the antibody reliable for its intended applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wojciech (Verified Customer) (05-31-2024) | WB at 1:1000. Two (80 and 104 kDa) bands detected.
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Wojciech (Verified Customer) (05-21-2024) | Used in WB and got 2 bands at 81 and 104 kDa at 1:1000 dilution.
|
Boyan (Verified Customer) (03-11-2019) | For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.
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Alex (Verified Customer) (10-23-2018) | Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.
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