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  • KD/KO Validated

FURIN Polyclonal antibody

FURIN Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 18413-1-AP

Synonyms

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1



Tested Applications

Positive WB detected inHepG2 cells, COLO 320 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18413-1-AP targets FURIN in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FURIN fusion protein Ag13261
Full Name furin (paired basic amino acid cleaving enzyme)
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 90-110 kDa
GenBank Accession NumberBC012181
Gene Symbol FURIN
Gene ID (NCBI) 5045
RRIDAB_2878542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The molecular weight of mature furin is 75-90 kDa and that of pre-pro furin is 95 kDa. With glycosylation and other post-translational modifications, the molecular weight of pre-pro furin will be migrated to 100-110 kDa. (PMID: 1644796, 1885622, 31336100, 35322530).

Protocols

Product Specific Protocols
WB protocol for FURIN antibody 18413-1-APDownload protocol
IHC protocol for FURIN antibody 18413-1-APDownload protocol
IP protocol for FURIN antibody 18413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Rep

Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury

Authors - Yiheng Mao
humanWB

Oncogene

Lipoic acid-induced oxidative stress abrogates IGF-1R maturation by inhibiting the CREB/furin axis in breast cancer cell lines.

Authors - Diana Farhat
humanWB

PLoS Pathog

Antibody-mediated spike activation promotes cell-cell transmission of SARS-CoV-2

Authors - Shi Yu
  • KD Validated
humanWB

Oncogenesis

Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.

Authors - Huizhi Wang
humanWB

Br J Cancer

Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.

Authors - Diana Farhat
  • KD Validated
humanWB,IF

J Virol

Interplay between Furin and Sialoglycans in Modulating Adeno-Associated Viral Cell Entry

Authors - Timothy J Smith
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Wojciech (Verified Customer) (05-31-2024)

WB at 1:1000. Two (80 and 104 kDa) bands detected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T-ALL cell lines
FH

Wojciech (Verified Customer) (05-21-2024)

Used in WB and got 2 bands at 81 and 104 kDa at 1:1000 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: T-ALL cell lines
FH

Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: MEF
FH

Alex (Verified Customer) (10-23-2018)

Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: 293T, HeLa