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  • KD/KO Validated

FURIN Polyclonal antibody

FURIN Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18413-1-AP

Synonyms

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1



Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18413-1-AP is FURIN Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FURIN fusion protein Ag13261
Full Name furin (paired basic amino acid cleaving enzyme)
Calculated molecular weight 87 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberBC012181
Gene symbol FURIN
Gene ID (NCBI) 5045
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.

Protocols

Product Specific Protocols
WB protocol for FURIN antibody 18413-1-APDownload protocol
IHC protocol for FURIN antibody 18413-1-APDownload protocol
IP protocol for FURIN antibody 18413-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors.

Authors - Xin Yu
humanWB

Oncol Lett

Furin inhibitor D6R suppresses epithelial-mesenchymal transition in SW1990 and PaTu8988 cells via the Hippo-YAP signaling pathway.

Authors - Meng Zhou
humanWB

Angiogenesis

SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization.

Authors - Jia-Xing Sun
humanWB

Br J Cancer

Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.

Authors - Diana Farhat
  • KD Validated
humanWB

Oncogenesis

Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.

Authors - Huizhi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (03-11-2019)

For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.

  • Applications: Western Blot,Immunofluorescence,
  • Cell Tissue Type: MEF
FH

Alex (Verified Customer) (10-23-2018)

Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: 293T, HeLa