Tested Applications
| Positive WB detected in | HepG2 cells, COLO 320 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 19 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
18413-1-AP targets FURIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FURIN fusion protein Ag13261 Predict reactive species |
| Full Name | furin (paired basic amino acid cleaving enzyme) |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 90-110 kDa |
| GenBank Accession Number | BC012181 |
| Gene Symbol | FURIN |
| Gene ID (NCBI) | 5045 |
| RRID | AB_2878542 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09958 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The molecular weight of mature furin is 75-90 kDa and that of pre-pro furin is 95 kDa. With glycosylation and other post-translational modifications, the molecular weight of pre-pro furin will be migrated to 100-110 kDa. (PMID: 1644796, 1885622, 31336100, 35322530).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FURIN antibody 18413-1-AP | Download protocol |
| IHC protocol for FURIN antibody 18413-1-AP | Download protocol |
| IF protocol for FURIN antibody 18413-1-AP | Download protocol |
| IP protocol for FURIN antibody 18413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Furin Egress from the TGN is Regulated by Membrane-Associated RING-CH Finger (MARCHF) Proteins and Ubiquitin-Specific Protease 32 (USP32) via Nondegradable K33-Polyubiquitination
| ||
Cell Rep Deep spatial proteomics reveals region-specific features of severe COVID-19-related pulmonary injury | ||
Oncogene Lipoic acid-induced oxidative stress abrogates IGF-1R maturation by inhibiting the CREB/furin axis in breast cancer cell lines. | ||
PLoS Pathog Antibody-mediated spike activation promotes cell-cell transmission of SARS-CoV-2
| ||
Oncogenesis Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells. | ||
Br J Cancer Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Wojciech (Verified Customer) (05-31-2024) | WB at 1:1000. Two (80 and 104 kDa) bands detected.
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FH Wojciech (Verified Customer) (05-21-2024) | Used in WB and got 2 bands at 81 and 104 kDa at 1:1000 dilution.
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FH Boyan (Verified Customer) (03-11-2019) | For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.
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FH Alex (Verified Customer) (10-23-2018) | Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.
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