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- KD/KO Validated
FURIN Polyclonal antibody
FURIN Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC,ELISA
Conjugate
Unconjugated
Cat no : 18413-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, COLO 320 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
Product Information
The immunogen of 18413-1-AP is FURIN Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | chicken, human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FURIN fusion protein Ag13261 |
| Full Name | furin (paired basic amino acid cleaving enzyme) |
| Calculated molecular weight | 87 kDa |
| Observed molecular weight | 90-110 kDa |
| GenBank accession number | BC012181 |
| Gene symbol | FURIN |
| Gene ID (NCBI) | 5045 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FURIN antibody 18413-1-AP | Download protocol |
| IHC protocol for FURIN antibody 18413-1-AP | Download protocol |
| IP protocol for FURIN antibody 18413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS One Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors. | ||
Oncol Lett Furin inhibitor D6R suppresses epithelial-mesenchymal transition in SW1990 and PaTu8988 cells via the Hippo-YAP signaling pathway. | ||
Front Microbiol Cellular CARD11 Inhibits the Fusogenic Activity of Newcastle Disease Virus via CBM Signalosome-Mediated Furin Reduction in Chicken Fibroblasts. | ||
Angiogenesis SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization. | ||
Br J Cancer Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.
| ||
Oncogenesis Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Boyan (Verified Customer) (03-11-2019) | For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.
|
FH Alex (Verified Customer) (10-23-2018) | Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.
|
