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FURIN Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 18413-1-AP

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Tested Applications

Applications:
WB, IP, IHC, ELISA
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Images See all

FURIN Antibody WB HeLa cells 18413-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 18413-1-AP( FURIN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

FURIN Antibody IP HeLa cells 18413-1-AP

IP Result of anti-FURIN (IP:18413-1-AP, 4ug; Detection:18413-1-AP 1:500) with HeLa cells lysate 1400ug.

FURIN Antibody IHC human placenta tissue 18413-1-AP

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 18413-1-AP( FURIN Antibody) at dilution of 1:200 (under 10x lens).

FURIN Antibody IHC human placenta tissue 18413-1-AP

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 18413-1-AP( FURIN Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, COLO 320 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human placenta tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
FURIN fusion protein Ag13261
Full name:
furin (paired basic amino acid cleaving enzyme)
Calculated molecular weight:
87 kDa
Observed molecular weight:
90 kDa
GenBank accession number:
BC012181
Gene ID (NCBI):
5045
Gene symbol
FURIN
Synonyms
Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1

Background

FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.

Customer Reviews

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Share your scientific insight on this antibody

FURIN Rabbit Polyclonal antibody

Alex Compton, 10-23-2018
Applications
Immunofluorescence,
Primary Antibody Dilution
1:200

Cell/Tissue Type
293T, HeLa
Lot Number
Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.

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