FURIN Monoclonal antibody

FURIN Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1B9

Cat no : 67481-1-Ig

Synonyms

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1



Tested Applications

Positive WB detected inA549 cells, JAR cells, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67481-1-Ig targets FURIN in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FURIN fusion protein Ag13313
Full Name furin (paired basic amino acid cleaving enzyme)
Calculated molecular weight 87 kDa
Observed molecular weight 90-110 kDa
GenBank accession numberBC012181
Gene symbol FURIN
Gene ID (NCBI) 5045
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.

Protocols

Product Specific Protocols
WB protocol for FURIN antibody 67481-1-IgDownload protocol
IHC protocol for FURIN antibody 67481-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols