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FURIN Monoclonal antibody

FURIN Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1B9

Cat no : 67481-1-Ig

Synonyms

Dibasic processing enzyme, FUR, FURIN, PACE, PCSK3, SPC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FURIN Monoclonal antibody (67481-1-Ig)

    WB analysis using 67481-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using FURIN Monoclonal antibody (67481-1-Ig)

    WB analysis of COLO 320 using 67481-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using FURIN Monoclonal antibody (67481-1-Ig)

    WB analysis of NIH/3T3 using 67481-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67481-1-Ig (FURIN antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FURIN Monoclonal antibody (67481-1-Ig)

    IHC staining of human placenta using 67481-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using FURIN Monoclonal antibody (67481-1-Ig)

    IHC staining of human placenta using 67481-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67481-1-Ig (FURIN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, COLO 320 cells, NIH/3T3 cells, JAR cells, HeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67481-1-Ig targets FURIN in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FURIN fusion protein Ag13313
Full Name furin (paired basic amino acid cleaving enzyme)
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 90-110 kDa
GenBank Accession NumberBC012181
Gene Symbol FURIN
Gene ID (NCBI) 5045
RRIDAB_2882708
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.

Protocols

Product Specific Protocols
WB protocol for FURIN antibody 67481-1-IgDownload protocol
IHC protocol for FURIN antibody 67481-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols