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FUS/TLS Monoclonal antibody
FUS/TLS Monoclonal Antibody for FC, IF, IHC, IP, ELISA
Cat no : 60160-1-Ig
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human gliomas tissue, human brain (FTLD) tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human brain(ALS) tissue|
|Positive FC detected in||K-562 cells|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60160-1-Ig targets FUS/TLS in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||Drosophila, human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||FUS/TLS fusion protein Ag2150|
|Full Name||fusion (involved in t(12;16) in malignant liposarcoma)|
|Calculated molecular weight||75 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC026062|
|Gene ID (NCBI)||2521|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases. There is some debate as to whether FUS colocalizes with TDP-43 in TDP-43-positive cases of ALS and whether TDP-43 and FUS cause neurodegenerative disease independently or contributively of one another. This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.
|Product Specific Protocols|
|WB protocol for FUS/TLS antibody 60160-1-Ig||Download protocol|
|IHC protocol for FUS/TLS antibody 60160-1-Ig||Download protocol|
|IP protocol for FUS/TLS antibody 60160-1-Ig||Download protocol|
|FC protocol for FUS/TLS antibody 60160-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Acta Neuropathol Commun
Topography of FUS pathology distinguishes late-onset BIBD from aFTLD-U.
Acta Neuropathol Commun
RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.
J Neuropathol Exp Neurol
Poly-A Binding Protein-1 Localization to a Subset of TDP-43 Inclusions in Amyotrophic Lateral Sclerosis Occurs More Frequently in Patients Harboring an Expansion in C9orf72.
J Biol Chem
Hu Antigen R (HuR) is a Positive Regulator of RNA Binding Proteins TDP-43 and FUS/TLS: Implications for Amyotrophic Lateral Sclerosis.
Hum Mol Genet
Self-assembly of FUS through its low-complexity domain contributes to neurodegeneration.
Hum Mol Genet
PINK1 and Parkin are genetic modifiers for FUS-induced neurodegeneration.