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  • KD/KO Validated

FUS/TLS Monoclonal antibody

FUS/TLS Monoclonal Antibody for FC, IF, IHC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3A10B5

Cat no : 60160-1-Ig

Synonyms

75 kDa DNA pairing protein, FUS, FUS CHOP, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP



Tested Applications

Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman brain(ALS) tissue
Positive FC detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60160-1-Ig targets FUS/TLS in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity Drosophila, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FUS/TLS fusion protein Ag2150
Full Name fusion (involved in t(12;16) in malignant liposarcoma)
Calculated molecular weight 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC026062
Gene symbol FUS
Gene ID (NCBI) 2521
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration. Two research groups simultaneously reported that FUS is present in 5% of the pathalogical aggregations (inclusions) seen in familial amyotrophic sclerosis (fALS). FUS-positive inclusions were also reported in cases of sporadic ALS (sALS). More recently, wild-type FUS has also been implicated in the pathological development of frototemporal lobar dementia (FTLD) with ubiquitin-positive inclusions (FTLD-U), further linking FUS to the pathogenesis of neurogenerative diseases. There is some debate as to whether FUS colocalizes with TDP-43 in TDP-43-positive cases of ALS and whether TDP-43 and FUS cause neurodegenerative disease independently or contributively of one another. This antibody is a mouse monoclonal antibody raised against an internal region of human FUS. Initial reports from our customers suggest this new monoclonal FUS antibody (60160-1-Ig) is a useful tool in ALS and FTLD research. For more details, please see our blog article regarding the matter.

Protocols

Product Specific Protocols
WB protocol for FUS/TLS antibody 60160-1-IgDownload protocol
IHC protocol for FUS/TLS antibody 60160-1-IgDownload protocol
IP protocol for FUS/TLS antibody 60160-1-IgDownload protocol
FC protocol for FUS/TLS antibody 60160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Acta Neuropathol Commun

Topography of FUS pathology distinguishes late-onset BIBD from aFTLD-U.

Authors - Lee Edward B EB
humanIF

Acta Neuropathol Commun

RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases.

Authors - Mahlon Collins

J Neuropathol Exp Neurol

Poly-A Binding Protein-1 Localization to a Subset of TDP-43 Inclusions in Amyotrophic Lateral Sclerosis Occurs More Frequently in Patients Harboring an Expansion in C9orf72.

Authors - Leeanne McGurk
humanWB

J Biol Chem

Hu Antigen R (HuR) is a Positive Regulator of RNA Binding Proteins TDP-43 and FUS/TLS: Implications for Amyotrophic Lateral Sclerosis.

Authors - Liang Lu
  • KD Validated
human

Hum Mol Genet

Self-assembly of FUS through its low-complexity domain contributes to neurodegeneration.

Authors - Taisei Matsumoto
DrosophilaWB

Hum Mol Genet

PINK1 and Parkin are genetic modifiers for FUS-induced neurodegeneration.

Authors - Yanbo Chen