|Positive WB detected in||human skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue, mouse kidney tissue, human brain tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1500|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13721-1-AP targets Phospholemman/FXYD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phospholemman/FXYD1 fusion protein Ag4669|
|Full Name||FXYD domain containing ion transport regulator 1|
|Calculated molecular weight||92 aa, 10 kDa|
|Observed molecular weight||10-15 kDa|
|GenBank accession number||BC032800|
|Gene ID (NCBI)||5348|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)
|Product Specific Protocols|
|WB protocol for Phospholemman/FXYD1 antibody 13721-1-AP||Download protocol|
|IHC protocol for Phospholemman/FXYD1 antibody 13721-1-AP||Download protocol|
|IP protocol for Phospholemman/FXYD1 antibody 13721-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Appl Physiol (1985)
Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men.
Am J Physiol Cell Physiol
Phospholemman is not required for the acute stimulation of Na,K-ATPase α2 activity during skeletal muscle fatigue.
J Appl Physiol (1985)
Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners.
Eur J Appl Physiol
Muscle variables of importance for physiological performance in competitive football.
Scand J Med Sci Sports
The effect of repeated periods of speed endurance training on performance, running economy and muscle adaptations (113/120 characters).
Am J Physiol Regul Integr Comp Physiol
Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes.