Phospholemman/FXYD1 Polyclonal antibody

Phospholemman/FXYD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13721-1-AP

Synonyms

FXYD1, Phospholemman, PLM



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue, mouse kidney tissue, human brain tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13721-1-AP targets Phospholemman/FXYD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rabbit, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Phospholemman/FXYD1 fusion protein Ag4669
Full Name FXYD domain containing ion transport regulator 1
Calculated molecular weight 92 aa, 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC032800
Gene symbol FXYD1
Gene ID (NCBI) 5348
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FXYD1, also named as PLM and Phospholemman, belongs to the FXYD family. FXYD1 induces a hyperpolarization-activated chloride current when expressed in Xenopus oocytes. It may have a functional role in muscle contraction. FXYD1 is a partner protein and regulator of the Na+,K+-ATPase (Na+,K+-pump). It may play a role in the acute regulation of the Na+,K+-ATPase response to exercise. (PMID: 20595385, 21653224)

Protocols

Product Specific Protocols
WB protocol for Phospholemman/FXYD1 antibody 13721-1-APDownload protocol
IHC protocol for Phospholemman/FXYD1 antibody 13721-1-APDownload protocol
IP protocol for Phospholemman/FXYD1 antibody 13721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Appl Physiol (1985)

Mechanisms underlying enhancements in muscle force and power output during maximal cycle ergometer exercise induced by chronic β2-adrenergic stimulation in men.

Authors - Morten Hostrup
WB

Am J Physiol Cell Physiol

Phospholemman is not required for the acute stimulation of Na,K-ATPase α2 activity during skeletal muscle fatigue.

Authors - Palanikumar Manoharan

J Appl Physiol (1985)

Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners.

Authors - Casper Skovgaard
humanWB

Eur J Appl Physiol

Muscle variables of importance for physiological performance in competitive football.

Authors - Magni Mohr
human

Scand J Med Sci Sports

The effect of repeated periods of speed endurance training on performance, running economy and muscle adaptations (113/120 characters).

Authors - Casper Skovgaard
WB

Am J Physiol Regul Integr Comp Physiol

Intensive training and reduced volume increases muscle FXYD1 expression and phosphorylation at rest and during exercise in athletes.

Authors - Martin Thomassen