Dysadherin Polyclonal antibody

Dysadherin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12166-1-AP

Synonyms

dysad, Dysadherin, FXYD5, HSPC113, IWU 1, IWU1, KCT1, OIT2, PRO6241, RIC, UNQ2561/PRO6241



Tested Applications

Positive WB detected inmouse small intestine tissue, human placenta tissue
Positive IHC detected inhuman kidney tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12166-1-AP targets Dysadherin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dysadherin fusion protein Ag2808
Full Name FXYD domain containing ion transport regulator 5
Calculated molecular weight 178 aa, 19 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC009642
Gene symbol FXYD5
Gene ID (NCBI) 53827
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dysadherin, also known as FXYD5 (FXYD domain containing ion transport regulator 5), is a cancer-associated cell membrane glycoprotein which belongs to the FXYD family. The FXYD family, which contains seven members, are tissue specific regulators of the Na,K-ATPase. Dysadherin is involved in down-regulation of E-cadherin, which plays important roles in tumor development and metastasis. It is present in spleen, lung, skeletal muscle, and testis. Increased expression of dysadherin has been associated with increased cell motility and metastatic potential.

Protocols

Product Specific Protocols
WB protocol for Dysadherin antibody 12166-1-APDownload protocol
IHC protocol for Dysadherin antibody 12166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Oncol

A FXYD5/TGF‑β/SMAD positive feedback loop drives epithelial‑to‑mesenchymal transition and promotes tumor growth and metastasis in ovarian cancer.

Authors - Yang Bai