Tested Applications
| Positive WB detected in | mouse small intestine tissue, human placenta tissue |
| Positive IHC detected in | human kidney tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12166-1-AP targets Dysadherin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2808 Product name: Recombinant human FXYD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-178 aa of BC009642 Sequence: KDTTSSSSADSTIMDIQVPTRAPDAVYTELQPTSPTPTWPADETPQPQTQTQQLEGTDGPLVTDPETHKSTKAAHPTDDTTTLSERPSPSTDVQTDPQTLKPSGFHEDDPFFYDEHTLRKRGLLVAAVLFITGIIILTSGKCRQLSRLCRNHCR Predict reactive species |
| Full Name | FXYD domain containing ion transport regulator 5 |
| Calculated Molecular Weight | 178 aa, 19 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC009642 |
| Gene Symbol | Dysadherin |
| Gene ID (NCBI) | 53827 |
| RRID | AB_10640583 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96DB9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dysadherin, also known as FXYD5 (FXYD domain containing ion transport regulator 5), is a cancer-associated cell membrane glycoprotein which belongs to the FXYD family. The FXYD family, which contains seven members, are tissue specific regulators of the Na,K-ATPase. Dysadherin is involved in down-regulation of E-cadherin, which plays important roles in tumor development and metastasis. It is present in spleen, lung, skeletal muscle, and testis. Increased expression of dysadherin has been associated with increased cell motility and metastatic potential.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Dysadherin antibody 12166-1-AP | Download protocol |
| WB protocol for Dysadherin antibody 12166-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-FXYD5 antibody (Cat# 12166-1-AP) has 10 total citations. These appear in journals including Int J Oncol, Cancer Gene Ther, iScience, Cell Transplant, Exp Cell Res, Biochimie, Mol Biotechnol, J Dev Biol, Bioengineered, and Transpl Immunol. Research fields span ovarian, gastric, thyroid, kidney, pancreatic, and lung cancers; EMT and metastasis; hematopoiesis; inflammatory responses; and cardiac ischemia/reperfusion injury. Applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Int J Oncol A FXYD5/TGF‑β/SMAD positive feedback loop drives epithelial‑to‑mesenchymal transition and promotes tumor growth and metastasis in ovarian cancer. | ||
Cancer Gene Ther FXYD5 regulates gastric cancer cell metastasis and drug resistance by EMT modulation
| ||
Cell Transplant Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer. | ||
Exp Cell Res USP11 stabilizes RALY to promote FXYD5-mediated aerobic glycolysis and aggravate pancreatic cancer progression | ||
Biochimie Generation of the novel anti-FXYD5 monoclonal antibody and its application to the diagnosis of pancreatic and lung cancer
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