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- KD/KO Validated
GARS Polyclonal antibody
GARS Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, RIP, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15831-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, HeLa cells, HepG2 cells, HT-1080 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| CoIP | See 2 publications below |
| RIP | See 1 publications below |
Product Information
15831-1-AP targets GARS in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, Drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GARS fusion protein Ag8322 |
| Full Name | glycyl-tRNA synthetase |
| Calculated Molecular Weight | 739 aa, 83 kDa |
| Observed Molecular Weight | 75-80 kDa |
| GenBank Accession Number | BC007755 |
| Gene Symbol | GARS |
| Gene ID (NCBI) | 2617 |
| RRID | AB_2294385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GARS(glycinamide ribonucleotide synthetase) perform an essential function in protein synthesis by catalyzing the esterification of an amino acid to its cognate tRNA.GARS, however, is a ubiquitously expressed α2 homodimer responsible for charging tRNA molecules with glycine, this enzyme is essential for protein synthesis in all cells and tissues(PMID:17035524 ).It can exist as a oligomer with 250KDa(PMID:22504945).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GARS antibody 15831-1-AP | Download protocol |
| IHC protocol for GARS antibody 15831-1-AP | Download protocol |
| IF protocol for GARS antibody 15831-1-AP | Download protocol |
| IP protocol for GARS antibody 15831-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Brain HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease.
| ||
Nat Commun Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. | ||
EMBO Rep The tRNA-like small noncoding RNA mascRNA promotes global protein translation.
| ||
Hum Mol Genet Mutations in glycyl-tRNA-synthetase impair mitochondrial metabolism in neurons.
|
