|Positive IHC detected in||human pancreas tissue, human small intestine tissue, human colon tissue, rat small intestine tissue, mouse small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human pancreas cancer tissue, human pancreas tissue, mouse pancreas tissue|
|Immunohistochemistry (IHC)||IHC : 1:12800-1:51200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15954-1-AP targets Glucagon in IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Glucagon fusion protein Ag8677|
|Calculated molecular weight||180 aa, 21 kDa|
|GenBank accession number||BC005278|
|Gene ID (NCBI)||2641|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glucagon is a protein cleaved from a precursor protein encoded by the GCG gene. The distinct peptides formed from this precursor include Glucagon, Glucagon-Like Peptide 1 (GLP-1), Glucagon-Like Peptide 2 (GLP-2), and oxyntomodulin. Glucagon is essential in maintaining blood glucose homeostasis and therefore has a key role in Type 1 and Type 2 diabetes.
What is the molecular weight of Glucagon?
21 kDa. Glucagon is composed of 180 amino acids and is a ligand for a specific G-protein coupled receptor (GPCR).
Where is it expressed?
Glucagon is a hormone secreted by the pancreas, in the α cells of the islets of Langerhans (PMID: 18880761). GLP-1, GLP-2, and oxyntomodulin are secreted by gut endocrine cells of the GI tract and also by selected neurons in the brain. These secreted proteins are transported to the target organ in plasma.
What is the function of glucagon?
The regulation of blood glucose levels is controlled by a balance of the hormones INS and glucagon in a negative feedback loop. A drop in blood glucose, or hypoglycemia, stimulates the release of glucagon. This initiates a cascade of signals, ultimately causing the conversion of stored glycogen into free glucose in a process called glycogenolysis, which takes place in liver and muscle cells. Glucagon can also stimulate gluconeogenesis in the liver, the process of forming glucose from non-carbohydrate sources such as amino acids (PMID: 13477815).
What diseases are associated with glucagon?
Glucagon is key in homeostasis of blood glucose and maintaining the balance between hyperglycaemia and hypoglycemia. It is therefore important in the study of Type 1 and Type 2 diabetes mellitus, where dysfunction of the pancreatic cells leads to a deregulation of blood glucose levels (PMID: 19326096). In both forms of the disease, glucagon secretion is impaired, from too much being secreted during hyperglycaemia, to too little being released to normalize hypoglycemia (PMID: 18197838).
aThe characteristics of glucose metabolism in the sulfonylurea receptor 1 knockout rat model.
SIRT6-mediated transcriptional suppression of Txnip is critical for pancreatic beta cell function and survival in mice.
Biochem Biophys Rep
Mint3 is dispensable for pancreatic and kidney functions in mice.
Methods Mol Biol
Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function.
The active GLP-1 analogue liraglutide alleviates H9N2 influenza virus-induced acute lung injury in mice.
Genetic activation of α-cell glucokinase in mice causes enhanced glucose-suppression of glucagon secretion during normal and diabetic states.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
kk (Verified Customer) (11-21-2018)
very strong and specific staining in mouse pancreas tissues