Glucagon Polyclonal antibody

Glucagon Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15954-1-AP

Synonyms

GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM, Proglucagon



Tested Applications

Positive IHC detected inhuman pancreas tissue, human small intestine tissue, human colon tissue, rat small intestine tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas cancer tissue, human pancreas tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:12800-1:51200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15954-1-AP targets Glucagon in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Glucagon fusion protein Ag8677
Full Name glucagon
Calculated molecular weight 180 aa, 21 kDa
GenBank accession numberBC005278
Gene symbol GCG
Gene ID (NCBI) 2641
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Background

Glucagon is a protein cleaved from a precursor protein encoded by the GCG gene. The distinct peptides formed from this precursor include Glucagon, Glucagon-Like Peptide 1 (GLP-1), Glucagon-Like Peptide 2 (GLP-2), and oxyntomodulin. Glucagon is essential in maintaining blood glucose homeostasis and therefore has a key role in Type 1 and Type 2 diabetes.

 What is the molecular weight of Glucagon?

21 kDa. Glucagon is composed of 180 amino acids and is a ligand for a specific G-protein coupled receptor (GPCR).

 Where is it expressed?

Glucagon is a hormone secreted by the pancreas, in the α cells of the islets of Langerhans (PMID: 18880761). GLP-1, GLP-2, and oxyntomodulin are secreted by gut endocrine cells of the GI tract and also by selected neurons in the brain. These secreted proteins are transported to the target organ in plasma.

 What is the function of glucagon?

The regulation of blood glucose levels is controlled by a balance of the hormones INS and glucagon in a negative feedback loop. A drop in blood glucose, or hypoglycemia, stimulates the release of glucagon. This initiates a cascade of signals, ultimately causing the conversion of stored glycogen into free glucose in a process called glycogenolysis, which takes place in liver and muscle cells. Glucagon can also stimulate gluconeogenesis in the liver, the process of forming glucose from non-carbohydrate sources such as amino acids (PMID: 13477815).

 What diseases are associated with glucagon?

Glucagon is key in homeostasis of blood glucose and maintaining the balance between hyperglycaemia and hypoglycemia. It is therefore important in the study of Type 1 and Type 2 diabetes mellitus, where dysfunction of the pancreatic cells leads to a deregulation of blood glucose levels (PMID: 19326096). In both forms of the disease, glucagon secretion is impaired, from too much being secreted during hyperglycaemia, to too little being released to normalize hypoglycemia (PMID: 18197838).


Protocols

Product Specific Protocols
IHC protocol for Glucagon antibody 15954-1-APDownload protocol
IF protocol for Glucagon antibody 15954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Mol Med

aThe characteristics of glucose metabolism in the sulfonylurea receptor 1 knockout rat model.

Authors - Xiaojun Zhou
mouseIHC

Diabetologia

SIRT6-mediated transcriptional suppression of Txnip is critical for pancreatic beta cell function and survival in mice.

Authors - Kunhua Qin
humanIF

Methods Mol Biol

Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca2+ Influx to Assess the Role of Potassium Channels on β-Cell Function.

Authors - Nicholas C Vierra

Sci Signal

TALK-1 channels control β cell endoplasmic reticulum Ca2+ homeostasis.

Authors - Nicholas C Vierra
mouseIF

Mol Metab

TALK-1 reduces delta-cell endoplasmic reticulum and cytoplasmic calcium levels limiting somatostatin secretion.

Authors - Nicholas C Vierra
humanIHC

Antioxid Redox Signal

PMID: 25556857

Authors - Hongzhi Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

kk (Verified Customer) (11-21-2018)

very strong and specific staining in mouse pancreas tissues

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse islets