Tested Applications
| Positive WB detected in | Jurkat cells, L02 cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, Ramos cells, mouse lung tissue, rat lung tissue, C6 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12601-1-AP targets GCLC in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, chicken, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3252 Product name: Recombinant human GCLC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-252 aa of BC022487 Sequence: MGLLSQGSPLSWEETKRHADHVRRHGILQFLHIYHAVKDRHKDVLKWGDEVEYMLVSFDHENKKVRLVLSGEKVLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEANMRKRRKEATSILEENQALCTITSFPRLGCPGFTLPEVKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFTEDDEASRASKPDHIYMDAMGFGMGNCCLQV Predict reactive species |
| Full Name | glutamate-cysteine ligase, catalytic subunit |
| Calculated Molecular Weight | 637 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC022487 |
| Gene Symbol | GCLC |
| Gene ID (NCBI) | 2729 |
| RRID | AB_2278734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48506 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family. It is also known as GLCL and GLCLC. It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis. GCLC may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GCLC antibody 12601-1-AP | Download protocol |
| IP protocol for GCLC antibody 12601-1-AP | Download protocol |
| WB protocol for GCLC antibody 12601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GCLC Polyclonal antibody (Cat# 12601-1-AP) has accumulated 206 citations across high-impact journals including Nature, Science, Cell, Nature Communications, Cell Metabolism, PNAS, Hepatology, and Cell Death & Differentiation. Research fields span ferroptosis, oxidative stress, Nrf2/Keap1 signaling, glutathione metabolism, liver injury, neurodegeneration, cancer biology, inflammation, and mitochondrial homeostasis. The antibody is predominantly used in Western blot across human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Nature SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.
| ||
Cell Metab Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex | ||
Cell Metab Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism. |
Reviews
What customers say
All three reviews rate this antibody 5 stars. Users consistently report sharp, clear, and crisp bands in Western Blot applications, with one noting a 1:5000 dilution in colorectal cancer samples. For IHC, one user highlighted a positive experience staining human pancreas and liver tissues, noting the antibody was recommended by their IHC core. Overall, reviewers express strong satisfaction with the antibody's specificity and performance across both WB and IHC.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sai Sindhura (Verified Customer) (01-08-2026) | Antibody is giving sharp bands
|
Zach (Verified Customer) (08-29-2025) | Our IHC core recommend me to get this antibody for IHC staining in human samples, pretty good experience.
|
AIK SENG (Verified Customer) (01-10-2023) | Clear & crisp bands
|
