GCLC Antibody 12601-1-AP

Tested Applications

Applications:

ELISA, IHC, IP, WB
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Jurkat cells were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human kidney using 12601-1-AP(GCLC antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human kidney using 12601-1-AP(GCLC antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-GCLC (IP:12601-1-AP, 3ug; Detection:12601-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

Species specificity:

human, mouse, rat

Positive WB detected in:

Jurkat cells, HEK-293 cells, Jurkat cells, L02 cells, mouse kidney tissue, mouse lung tissue

Positive IP detected in:

mouse kidney tissue

Positive IHC detected in:

human kidney tissue

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:20-1:200

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

GCLC fusion protein Ag3252

Full name:

glutamate-cysteine ligase, catalytic subunit

Calculated molecular weight:

637aa,73 kDa

Observed molecular weight:

73 kDa

GenBank accession number:

BC022487

Gene ID (NCBI):

2729

Gene symbol

GCLC

Synonyms

Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC

Background

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

Showing 1- [6] of [11] Publications

Author:		Scannevin Robert H RH
Pubmed ID:		22267202
Journal:		J Pharmacol Exp Ther
Title:		Fumarates promote cytoprotection of central nervous system cells against oxidative stress via the nuclear factor (erythroid-derived 2)-like 2 pathway.
Application:		WB

Species:		human

Author:		Zhai Xiaohan X
Pubmed ID:		23255485
Journal:		Mol Nutr Food Res
Title:		Dietary flavonoid genistein induces Nrf2 and phase II detoxification gene expression via ERKs and PKC pathways and protects against oxidative stress in Caco-2 cells.
Application:		WB

Species:		human

Author:		Wu Hongtan H
Pubmed ID:		23684612
Journal:		Cell Rep
Title:		The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.
Application:		WB

Species:		mouse

Author:		Qiwang Ma
Pubmed ID:		25394658
Journal:		Am J Physiol Gastrointest Liver Physiol
Title:		Estradiol decreases taurine level by reducing cysteine sulfinic acid decarboxylase via the estrogen receptor-alpha in female mice liver.
Application:		WB

Species:		human

Author:		Ana Tomasovic
Pubmed ID:		25716320
Journal:		J Biol Chem
Title:		Sestrin 2 Regulates PDGF Receptor beta (PDGFRβ) Expression by Modulating Proteasomal and Nrf2 Transcription Factor Functions.
Application:		WB

Species:		human, mouse

Author:		Xiao-Hua Yuan
Pubmed ID:		26462682
Journal:		J Steroid Biochem Mol Biol
Title:		Progesterone amplifies oxidative stress signal and promotes NO production via H2O2 in mouse kidney arterial endothelial cells.
Application:		WB

Species:		mouse

WB Figures

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

L02 cells were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse lung tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse lung tissue were subjected to SDS PAGE followed by western blot with 12601-1-AP(GCLC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human kidney using 12601-1-AP(GCLC antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human kidney using 12601-1-AP(GCLC antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-GCLC (IP:12601-1-AP, 3ug; Detection:12601-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

GCLC Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 12601-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [6] of [11] Publications

WB Figures

IHC Figures

IP Figures

GCLC Antibody 11 Publications

Rabbit Polyclonal| Catalog number: 12601-1-AP

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Tested Applications

Product Information

Immunogen Information

Background