GCLC Polyclonal antibody

GCLC Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12601-1-AP

Synonyms

Gamma ECS, GCLC, GCS, GCS heavy chain, GLCL, GLCLC



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, L02 cells, mouse kidney tissue, mouse lung tissue, Raji cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12601-1-AP targets GCLC in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCLC fusion protein Ag3252
Full Name glutamate-cysteine ligase, catalytic subunit
Calculated molecular weight 637 aa, 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC022487
Gene symbol GCLC
Gene ID (NCBI) 2729
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCLC (Glutamate--cysteine ligase catalytic subunit) belongs to the glutamate--cysteine ligase type 3 family.It is also named as GLCL, GLCLC.It is the first rate-limiting enzyme in glutathione (GSH) biosynthesis.GCLC) gene may be associated with coronary endothelial vasomotor dysfunction and myocardial infarction (MI) (PMID:12598062). Defects in GCLC are the cause of hemolytic anemia (HAGGSD).

Protocols

Product Specific Protocols
WB protocol for GCLC antibody 12601-1-APDownload protocol
IHC protocol for GCLC antibody 12601-1-APDownload protocol
IP protocol for GCLC antibody 12601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Sci Food Agric

l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.

Authors - Zhengxuan Wang
ratWB

Front Mol Neurosci

SIRT2, ERK and Nrf2 Mediate NAD+ Treatment-Induced Increase in the Antioxidant Capacity of PC12 Cells Under Basal Conditions.

Authors - Jie Zhang
humanWB

Acta Biochim Biophys Sin (Shanghai)

High glucose inhibits vascular endothelial Keap1/Nrf2/ARE signal pathway via downregulation of monomethyltransferase SET8 expression.

Authors - Xiangyuan Chen
humanWB

Cell Death Dis

Computational repositioning of dimethyl fumarate for treating alcoholic liver disease.

Authors - Ye Zhang
mouseWB

J Steroid Biochem Mol Biol

Progesterone amplifies oxidative stress signal and promotes NO production via H2O2 in mouse kidney arterial endothelial cells.

Authors - Xiao-Hua Yuan
humanWB

Food Chem Toxicol

2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone, a potent Nrf2/ARE pathway inhibitor, reverses drug resistance by decreasing glutathione synthesis and drug efflux in BEL-7402/5-FU cells.

Authors - Xing Wei