Connexin-32 Polyclonal antibody

Connexin-32 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10450-1-AP

Synonyms

CMTX, CMTX1, Connexin-32, CX32, Gap junction beta 1 protein, GJB1



Tested Applications

Positive WB detected inmouse brain tissue, A375 cells, human liver tissue, mouse skin tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10450-1-AP targets Connexin-32 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Connexin-32 fusion protein Ag0659
Full Name gap junction protein, beta 1, 32kDa
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002805
Gene symbol GJB1
Gene ID (NCBI) 2705
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Connexin-32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-32 is the major gap junction protein of liver, and the expression is also found in brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin-32 plays an important role in peripheral nerve. Defects in Connexin-32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. (PMID: 8266101; 8613761; 8608591; 19265674)

Protocols

Product Specific Protocols
WB protocol for Connexin-32 antibody 10450-1-APDownload protocol
IHC protocol for Connexin-32 antibody 10450-1-APDownload protocol
IP protocol for Connexin-32 antibody 10450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Expression of connexin 32 and connexin 43 in acute myeloid leukemia and their roles in proliferation.

Authors - Yi Sha S
humanWB

Oncol Lett

Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.

Authors - Lihua Huang
humanIHC, IF

Histopathology

Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features.

Authors - Wen-Qun Zhong
humanIHC

J Biomed Biotechnol

Ischemia alters the expression of connexins in the aged human brain.

Authors - Nakase Taizen T
mouseWB

Brain Res Bull

Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice.

Authors - Chen Men
humanWB

World J Gastroenterol

PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis.

Authors - Xiao-Ming Liu