KGA/GAM/GAC Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 23549-1-AP

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Freight/Packing

Con: 74 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
HEK-293 cells, HEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human kidney tissue, human brain tissue, human kidney tissue

Positive IF detected in:
HEK-293 cells, HeLa cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
glutaminase

Calculated molecular weight:
669aa,73 kDa

Observed molecular weight:
58 kDa, 83 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
GLS

Synonyms
AAD20, DKFZp686O15119, FLJ10358, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KIAA0838, L glutamine amidohydrolase
Background

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody is specific to the 3 isoforms(KGA,GAM,GAC) of GLS.


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