GLS Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66265-1-Ig |CloneNo.: 3A12A1

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 150 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
human, pig

Positive WB detected in:
fetal human brain tissue, HEK-293 cells, rat liver tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:5000-1:50000
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2b

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
glutaminase

Calculated molecular weight:
669aa,73 kDa

Observed molecular weight:
58 kDa, 65 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
GLS

Synonyms
AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KIAA0838, L glutamine amidohydrolase
Background

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC.


Back
to top