|Positive WB detected in||mouse brain tissue, human heart tissue, HepG2 cells, L02 cells, mouse skeletal muscle tissue, human skeletal muscle tissue, rat brain tissue, human brain tissue, mouse liver tissue, mouse heart tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||L02 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14886-1-AP targets GOT1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GOT1 fusion protein Ag6671|
|Full Name||glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||43-46 kDa|
|GenBank accession number||BC000498|
|Gene ID (NCBI)||2805|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.
Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.
Am J Respir Cell Mol Biol
Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.
Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.
Mol Med Rep
Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.
Enhanced synthesis and diminished degradation of hydrogen sulfide in experimental colitis: a site-specific, pro-resolution mechanism.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Daniel (Verified Customer) (04-29-2019)
Strong band of the expected size. Weaker non-specific bands.