• Featured Product
  • KD/KO Validated

GOT1 Polyclonal antibody

GOT1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14886-1-AP

Synonyms

AATC, CAT, GIG18, GOT1, Transaminase A



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, HepG2 cells, L02 cells, mouse skeletal muscle tissue, human skeletal muscle tissue, rat brain tissue, human brain tissue, mouse liver tissue, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14886-1-AP targets GOT1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOT1 fusion protein Ag6671
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Calculated molecular weight 46 kDa
Observed molecular weight 43-46 kDa
GenBank accession numberBC000498
Gene symbol GOT1
Gene ID (NCBI) 2805
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

Protocols

Product Specific Protocols
WB protocol for GOT1 antibody 14886-1-APDownload protocol
IF protocol for GOT1 antibody 14886-1-APDownload protocol
IP protocol for GOT1 antibody 14886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Coordinative metabolism of glutamine carbon and nitrogen in proliferating cancer cells under hypoxia.

Authors - Yuanyuan Wang
humanWB

Am J Respir Cell Mol Biol

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Authors - Robert B Hamanaka
  • KD Validated
humanWB

Cell Rep

Glutamine Anabolism Plays a Critical Role in Pancreatic Cancer by Coupling Carbon and Nitrogen Metabolism.

Authors - Alex J Bott
mouseWB

Cell Rep

PMID: 31801093

Authors - Benjamin D Stein
ratWB

Mol Med Rep

Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats.

Authors - Maojun Liu
ratWB

PLoS One

Enhanced synthesis and diminished degradation of hydrogen sulfide in experimental colitis: a site-specific, pro-resolution mechanism.

Authors - Flannigan Kyle L KL

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Daniel (Verified Customer) (04-29-2019)

Strong band of the expected size. Weaker non-specific bands.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HK2 and RCC4 (human renal cells)
GOT1 Antibody Western Blot, validation (1:1000 dilution) in HK2 and RCC4 (human renal cells) (Cat no:14886-1-AP)