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GPR22 Polyclonal antibody

GPR22 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19951-1-AP

Synonyms

G protein coupled receptor 22, GPR22

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using GPR22 Polyclonal antibody (19951-1-AP)

    WB analysis of mouse heart using 19951-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using GPR22 Polyclonal antibody (19951-1-AP)

    WB analysis of rat brain using 19951-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GPR22 Polyclonal antibody (19951-1-AP)

    WB analysis of mouse brain using 19951-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19951-1-AP (GPR22 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using GPR22 Polyclonal antibody (19951-1-AP)

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GPR22 Polyclonal antibody (19951-1-AP)

    IHC staining of human testis using 19951-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19951-1-AP (GPR22 Antibody) at dilution of 1:100 (under 40x lens).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using GPR22 Polyclonal antibody (19951-1-AP)

    FC experiment of SH-SY5Y using 19951-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug GPR22 antibody (19951-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse heart tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19951-1-AP targets GPR22 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name G protein-coupled receptor 22
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberNM_005295
Gene Symbol GPR22
Gene ID (NCBI) 2845
RRIDAB_10644129
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR22 Belongs to the G-protein coupled receptor 1 family. GPR22 is an Orphan receptor. The antibody recognize the C-term of GPR22.

Protocols

Product Specific Protocols
WB protocol for GPR22 antibody 19951-1-APDownload protocol
IHC protocol for GPR22 antibody 19951-1-APDownload protocol
FC protocol for GPR22 antibody 19951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols