GPR162 Polyclonal antibody

GPR162 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15254-1-AP

Synonyms

A 2, G protein coupled receptor 162, GPR162, GRCA

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 15254-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 15254-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15254-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15254-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15254-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15254-1-AP (GPR162 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, human brain tissue, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

15254-1-AP targets GPR162 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR162 fusion protein Ag7011
• Full Name: G protein-coupled receptor 162
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 33 kDa, 64 kDa
• GenBank Accession Number: BC002353
• Gene Symbol: GPR162
• Gene ID (NCBI): 27239
• RRID: AB_10665369
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Acta Neuropathol Commun; Blood-based CNS regionally and neuronally enriched extracellular vesicles carrying pTau217 for Alzheimer's disease diagnosis and differential diagnosis; Authors - Zhen Guo